Cell-Laden and Cell-Free Matrix-Induced Chondrogenesis versus Microfracture for the Treatment of Articular Cartilage Defects: A Histological and Biomechanical Study in Sheep.
Cell-Laden and Cell-Free Matrix-Induced Chondrogenesis versus Microfracture for the Treatment of Articular Cartilage Defects: A Histological and Biomechanical Study in Sheep.
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DOI：
10.1177/1947603509358721
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发表时间：
2010-01
期刊：
影响因子：
2.8
通讯作者：
Kurz B
中科院分区：
文献类型：
--
作者：
Gille J;Kunow J;Boisch L;Behrens P;Bos I;Hoffmann C;Köller W;Russlies M;Kurz B
本研究的目的是评估再生潜力的细胞负载和无细胞的胶原蛋白基质相比，微骨折治疗应用于全层软骨缺损的绵羊模型。将动物（n = 30）随机分为5个治疗组，在两个膝关节的股骨托和内侧髁处设置7-mm的全软骨厚度缺损，并如下处理：（胶原蛋白I/III，Chondro-Gide®;胶原蛋白II，Chondrocell®），用于覆盖微骨折缺损（自体基质诱导的软骨发生），两种支架在体外用自体软骨细胞定殖（基质相关软骨细胞移植），或无支架微骨折技术。术后一年，对软骨损伤进行生物力学（压痕试验）、组织学（O 'Drivel评分）和免疫化学（I型和II型胶原染色）评价。与对照组相比，动物模型的所有治疗组诱导了更多的修复组织，并显示出更好的组织学评分和生物力学特性。当使用支架时，修复组织的平均厚度显著更大，尤其是胶原蛋白I/III膜。然而，从生物力学角度或基于组织学评分，没有一种首次手术优于其他手术。II型胶原蛋白的表达更好的髁状突缺损相比，trophila，尤其是在那些治疗与胶原蛋白I/III膜。用合适的基质覆盖缺损促进修复组织形成，并被认为是软骨缺损的有希望的治疗选择。然而，在给定的情况下，在绵羊模型中，与单独的微骨折相比，它未能改善再生关节软骨的生物力学和组织学特性。
The aim of this study was to evaluate the regenerative potential of cell-laden and cell-free collagen matrices in comparison to microfracture treatment applied to full-thickness chondral defects in an ovine model. Animals (n = 30) were randomized into 5 treatment groups, and 7-mm full-cartilage-thickness defects were set at the trochlea and medial condyle of both knee joints and treated as follows: 2 scaffolds in comparison (collagen I/III, Chondro-Gide®; collagen II, Chondrocell®) for covering microfractured defects (autologous matrix-induced chondrogenesis), both scaffolds colonized in vitro with autologous chondrocytes (matrix-associated chondrocyte transplantation), or scaffold-free microfracture technique. One year after surgery, cartilage lesions were biomechanically (indentation test), histologically (O’Driscoll score), and immunohistochemically (collagen type I and II staining) evaluated. All treatment groups of the animal model induced more repair tissue and showed better histological scores and biomechanical properties compared to controls. The average thickness of the repair tissue was significantly greater when a scaffold was used, especially the collagen I/III membrane. However, none of the index procedures surpassed the others from a biomechanical point of view or based on the histological scoring. Collagen type II expression was better in condylar defects compared to the trochlea, especially in those treated with collagen I/III membranes. Covering of defects with suitable matrices promotes repair tissue formation and is suggested to be a promising treatment option for cartilage defects. However, it failed to improve the biomechanical and histological properties of regenerated articular cartilage compared to microfracture alone in an ovine model under the given circumstances.
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DOI：
10.1515/bmt.2008.007
发表时间：
2008-01-01
期刊：
BIOMEDIZINISCHE TECHNIK
影响因子：
--
作者：
Koeller, Wolfgang;Kunow, Julius;Russlies, Martin
通讯作者：
Russlies, Martin
影响因子：
1.9
作者：
Behrens, Peter;Bitter, Thomas;Russlies, Martin
通讯作者：
Russlies, Martin
DOI：
10.1097/01.blo.0000164298.63534.64
发表时间：
2005-07-01
影响因子：
4.2
作者：
Browne, JE;Anderson, AF;Erggelet, C
通讯作者：
Erggelet, C
影响因子：
14
作者：
Buma, P;Schreurs, W;Verdonschot, N
通讯作者：
Verdonschot, N
影响因子：
4.8
作者：
Gill, TJ;McCulloch, PC;Morris, EA
通讯作者：
Morris, EA