Synergistic effect of CART (cocaine- and amphetamine-regulated transcript) peptide and cholecystokinin on food intake regulation in lean mice.
Synergistic effect of CART (cocaine- and amphetamine-regulated transcript) peptide and cholecystokinin on food intake regulation in lean mice.
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DOI：
10.1186/1471-2202-9-101
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发表时间：
2008-10-21
期刊：
影响因子：
2.4
通讯作者：
Zelezná B
中科院分区：
文献类型：
--
作者：
Maletínská L;Maixnerová J;Matysková R;Haugvicová R;Pirník Z;Kiss A;Zelezná B
CART（可卡因和安非他明调节转录物）肽和胆囊收缩素 (CCK) 是参与进食行为的神经调节剂。这项研究基于之前发现的瘦素和 CCK 对食物摄入的协同作用，以及我们对 CART 肽和 CCK 在食物摄入调节和 Fos 激活方面的合作的假设，这些目标是脑干孤束核 (NTS)、室旁核 (PVN) 和下丘脑背内侧核 (DMH)。在禁食的C57BL/6小鼠中，0.1或0.5μg/小鼠剂量的CART(61-102)的厌食作用被低剂量0.4或4μg/kg的CCK-8显着增强，而1mg/kg剂量的CCK-A受体拮抗剂devazepide阻断了CART(61-102)对食物摄入的影响。同时给予0.1μg/小鼠CART(61-102)和4μg/kg CCK-8后，NTS、PVN和DMH中Fos阳性神经元的数量显着高于给予每种特定肽后。此外，CART(61-102)和CCK-8在旷场试验中显示出对小鼠运动活性抑制的相加作用。 CART 肽和 CCK 对食物摄入的协同和持久作用以及它们对共同目标中 Fos 免疫反应性的累加效应表明 CART 肽和 CCK 的协同作用，这可能与瘦素对 CCK 饱腹感的协同作用有关。
CART (cocaine- and amphetamine-regulated transcript) peptide and cholecystokinin (CCK) are neuromodulators involved in feeding behavior. This study is based on previously found synergistic effect of leptin and CCK on food intake and our hypothesis on a co-operation of the CART peptide and CCK in food intake regulation and Fos activation in their common targets, the nucleus tractus solitarii of the brainstem (NTS), the paraventricular nucleus (PVN), and the dorsomedial nucleus (DMH) of the hypothalamus. In fasted C57BL/6 mice, the anorexigenic effect of CART(61-102) in the doses of 0.1 or 0.5 μg/mouse was significantly enhanced by low doses of CCK-8 of 0.4 or 4 μg/kg, while 1 mg/kg dose of CCK-A receptor antagonist devazepide blocked the effect of CART(61-102) on food intake. After simultaneous administration of 0.1 μg/mouse CART(61-102) and of 4 μg/kg of CCK-8, the number of Fos-positive neurons in NTS, PVN, and DMH was significantly higher than after administration of each particular peptide. Besides, CART(61-102) and CCK-8 showed an additive effect on inhibition of the locomotor activity of mice in an open field test. The synergistic and long-lasting effect of the CART peptide and CCK on food intake and their additive effect on Fos immunoreactivity in their common targets suggest a co-operative action of CART peptide and CCK which could be related to synergistic effect of leptin on CCK satiety.
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影响因子：
2.9
作者：
Kask, A;Schiöth, HB;Rägo, L
通讯作者：
Rägo, L
影响因子：
4.8
作者：
Merino, Beatriz;Cano, Victoria;Ruiz-Gayo, Mariano
通讯作者：
Ruiz-Gayo, Mariano
影响因子：
--
作者：
Cowles, RA;Segura, BJ;Mulholland, MW
通讯作者：
Mulholland, MW
DOI：
10.1046/j.1467-789x.2000.00007.x
发表时间：
2000-05-01
期刊：
Obesity reviews : an official journal of the International Association for the Study of Obesity
影响因子：
--
作者：
McMinn, J E;Baskin, D G;Schwartz, M W
通讯作者：
Schwartz, M W
影响因子：
2.9
作者：
Monnikes, H;Lauer, G;Arnold, R
通讯作者：
Arnold, R