Virus factories, double membrane vesicles and viroplasm generated in animal cells.
Virus factories, double membrane vesicles and viroplasm generated in animal cells.
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DOI：
10.1016/j.coviro.2011.09.008
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发表时间：
2011-11
影响因子：
5.9
通讯作者：
Wileman T
中科院分区：
文献类型：
--
作者：
Netherton CL;Wileman T
（+）链RNA病毒复制酶蛋白的装配发生在膜结合细胞器的细胞质表面。复制酶组装导致膜内陷，称为小球或双膜囊泡。磷脂酰肌醇-4-激酶-III的Arf 1依赖性募集可促进Arf 1复制酶组装。大的DNA病毒在核周工厂中复制和/或组装，类似于响应蛋白质聚集而产生的细胞攻击体。病毒工厂分离病毒DNA和RNA，似乎是细胞内不同的实体。许多病毒重组细胞膜区室和细胞骨架，以产生称为病毒工厂或“病毒质”的亚细胞微环境。这些创建了一个平台来浓缩复制所需的复制酶蛋白、病毒基因组和宿主蛋白，并保护免受抗病毒防御。人们越来越有兴趣了解病毒如何诱导细胞组织发生如此大的变化，最近的研究开始揭示病毒工厂和病毒质与容纳它们的细胞结构之间的关系。在这篇综述中，我们讨论了三个超组的（+）RNA病毒如何产生复制位点的膜结合细胞器和核质大DNA病毒诱导的核周工厂的重点研究。
► Assembly of replicase proteins of (+) strand RNA virus occurs on the cytoplasmic face of membrane-bound organelles. ► Replicase assembly leads to membrane invaginations called spherules or double membraned vesicles. ► Replicase assembly can be facilitated by Arf1 dependent recruitment of phosphatidylinositol-4-kinase-III. ► Large DNA viruses replicate and/or assemble in perinuclear factories that resemble cellular aggresomes generated in response to protein aggregation. ► Virus factories segregate viral DNA and RNA and appear to be distinct entities within the cell. Many viruses reorganise cellular membrane compartments and the cytoskeleton to generate subcellular microenvironments called virus factories or ‘viroplasm’. These create a platform to concentrate replicase proteins, virus genomes and host proteins required for replication and also protect against antiviral defences. There is growing interest in understanding how viruses induce such large changes in cellular organisation, and recent studies are beginning to reveal the relationship between virus factories and viroplasm and the cellular structures that house them. In this review, we discuss how three supergroups of (+)RNA viruses generate replication sites from membrane-bound organelles and highlight research on perinuclear factories induced by the nucleocytoplasmic large DNA viruses.
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影响因子：
6.7
作者：
Castelló A;Quintas A;Sánchez EG;Sabina P;Nogal M;Carrasco L;Revilla Y
通讯作者：
Revilla Y
DOI：
10.1083/jcb.153.3.449
发表时间：
2001-04-30
期刊：
The Journal of cell biology
影响因子：
--
作者：
Heath CM;Windsor M;Wileman T
通讯作者：
Wileman T
影响因子：
3.8
作者：
Monaghan, P;Cook, H;Wileman, T
通讯作者：
Wileman, T
影响因子：
5.4
作者：
Moffat, Katy;Knox, Caroline;Wileman, Thomas
通讯作者：
Wileman, Thomas
影响因子：
3.7
作者：
Fontana J;López-Iglesias C;Tzeng WP;Frey TK;Fernández JJ;Risco C
通讯作者：
Risco C