Separated transcriptomes of male gametophyte and tapetum in rice: validity of a laser microdissection (LM) microarray.
Separated transcriptomes of male gametophyte and tapetum in rice: validity of a laser microdissection (LM) microarray.
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大米中男配子体和tape虫的分离转录组：激光显微解剖（LM）微阵列的有效性。
DOI：
10.1093/pcp/pcn124
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发表时间：
2008-10
影响因子：
4.9
通讯作者：
Watanabe, Masao
中科院分区：
文献类型：
--
作者：
Suwabe, Keita;Suzuki, Go;Takahashi, Hirokazu;Shiono, Katsuhiro;Endo, Makoto;Yano, Kentaro;Fujita, Masahiro;Masuko, Hiromi;Saito, Hiroshi;Fujioka, Tomoaki;Kaneko, Fumi;Kazama, Tomohiko;Mizuta, Yoko;Kawagishi-Kobayashi, Makiko;Tsutsumi, Nobuhiro;Kurata, Nori;Nakazono, Mikio;Watanabe, Masao
在开花植物中，雄配子体，即花粉，在花药中发育。花药的配子体和孢子体组织中基因表达的复杂模式调节这一过程。小孢子/花粉和孢子体绒毡层的基因表达谱是特别感兴趣的。本研究采用激光微切割技术结合基因芯片技术（44 KLM-微芯片），对水稻小孢子/花粉和绒毡层的转录组进行了分析。11个已知的绒毡层特异基因的表达谱与以前的报道一致。根据其时空表达模式，将140个花药特异表达基因进一步分为雄配子体特异表达基因（71个，占51%）、绒毡层特异表达基因（7个，占5%）和雄配子体和绒毡层同时表达基因（62个，占44%）。这些结果表明，44 K LM-微阵列是一个可靠的工具，分析两个重要的细胞类型的花药，小孢子/花粉和绒毡层的基因表达谱。
In flowering plants, the male gametophyte, the pollen, develops in the anther. Complex patterns of gene expression in both the gametophytic and sporophytic tissues of the anther regulate this process. The gene expression profiles of the microspore/pollen and the sporophytic tapetum are of particular interest. In this study, a microarray technique combined with laser microdissection (44K LM-microarray) was developed and used to characterize separately the transcriptomes of the microspore/pollen and tapetum in rice. Expression profiles of 11 known tapetum specific-genes were consistent with previous reports. Based on their spatial and temporal expression patterns, 140 genes which had been previously defined as anther specific were further classified as male gametophyte specific (71 genes, 51%), tapetum-specific (seven genes, 5%) or expressed in both male gametophyte and tapetum (62 genes, 44%). These results indicate that the 44K LM-microarray is a reliable tool to analyze the gene expression profiles of two important cell types in the anther, the microspore/pollen and tapetum.
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影响因子：
7.4
作者：
Dembinsky, Diana;Woll, Katrin;Hochholdinger, Frank
通讯作者：
Hochholdinger, Frank
影响因子：
5.1
作者：
Jeon, JS;Chung, YY;An, GH
通讯作者：
An, GH
影响因子：
4.9
作者：
Hirano K;Aya K;Hobo T;Sakakibara H;Kojima M;Shim RA;Hasegawa Y;Ueguchi-Tanaka M;Matsuoka M
通讯作者：
Matsuoka M
影响因子：
11.6
作者：
KOLTUNOW, AM;TRUETTNER, J;GOLDBERG, RB
通讯作者：
GOLDBERG, RB
影响因子：
7.4
作者：
Honys, D;Twell, D
通讯作者：
Twell, D