Induced hepatic stem cells are suitable for human hepatocyte production.
Induced hepatic stem cells are suitable for human hepatocyte production.
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诱导的肝干细胞适用于人肝细胞的产生。
DOI：
10.1016/j.isci.2022.105052
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发表时间：
2022-10-21
期刊：
影响因子：
5.8
通讯作者：
Noguchi, Hirofumi
中科院分区：
文献类型：
--
作者：
Nakashima, Yoshiki;Miyagi-Shiohira, Chika;Saitoh, Issei;Watanabe, Masami;Matsushita, Masayuki;Tsukahara, Masayoshi;Noguchi, Hirofumi
用表达OCT 3/4、SOX 2、KLF 4和C-MYC的仙台病毒载体（SeV）转染人肝细胞以产生肝细胞衍生的诱导多能干细胞（iPSC）。检查48个建立的肝细胞衍生的iPSC样集落中未分化标志物（第19-21代）和肝细胞特异性标志物（HSM）（第0-20代）的信使RNA（mRNA）表达。在48个克隆中，10个克隆在0-20代持续表达HNF 1 β和HNF 4 α。表达HSM的集落（iTS-L细胞：来自肝脏的诱导组织特异性干细胞）在微阵列和甲基化分析中显示出与成纤维细胞来源的iPSC（品系：201 B7）不同的趋势。iTS-L细胞在小鼠中形成畸胎瘤的可能性低于iPSC（He）。iTS-L细胞比iPSC（He）或iPSC（201 B7）更有效地分化成肝细胞样细胞。这些数据表明表达0 CT 3/4、S 0X 2、KLF 4和C-MYC的SeV诱导iPSC和iTS-L细胞的产生。iTS细胞具有自我更新和多能性iTS细胞表达组织特异性标志物iTS-L细胞比iPSC更不容易形成畸胎瘤人类;生物科学;细胞生物学;干细胞研究;发育生物学
Human hepatocytes were transfected with Sendai virus vectors (SeV) expressing OCT3/4, SOX2, KLF4, and C-MYC to produce hepatocyte-derived induced pluripotent stem cells (iPSCs). The messenger RNA (mRNA) expression of undifferentiated markers (passage 19-21) and hepatocyte-specific markers (HSMs) (passage 0-20) in 48 established hepatocyte-derived iPSC-like colonies was examined. Among the 48 clones, 10 clones continuously expressed HSM mRNA (HNF1β and HNF4α) in passage 0-20. The colonies which expressed HSMs (iTS-L cells: induced tissue-specific stem cells from liver) showed a different tendency in microarray and methylation analyses to fibroblast-derived iPSCs (strain: 201B7). iTS-L cells were less likely to form teratomas in mice than iPSCs (He). The iTS-L cells were differentiated into hepatocyte-like cells more efficiently than iPSCs (He) or iPSCs (201B7). These data suggest that SeV expressing OCT3/4, SOX2, KLF4, and C-MYC induce the generation of iPSCs and iTS-L cells. iTS cells have self-renewal and multipotency iTS cells express tissue-specific markers iTS-L cells are less prone to teratoma formation than iPSCs Human; Biological sciences; Cell biology; Stem cells research; Developmental biology
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影响因子：
8.8
作者：
Ang LT;Tan AKY;Autio MI;Goh SH;Choo SH;Lee KL;Tan J;Pan B;Lee JJH;Lum JJ;Lim CYY;Yeo IKX;Wong CJY;Liu M;Oh JLL;Chia CPL;Loh CH;Chen A;Chen Q;Weissman IL;Loh KM;Lim B
通讯作者：
Lim B
影响因子：
64.5
作者：
Takahashi, Kazutoshi;Tanabe, Koji;Yamanaka, Shinya
通讯作者：
Yamanaka, Shinya
DOI：
10.2183/pjab.85.348
发表时间：
2009
期刊：
Proceedings of the Japan Academy. Series B, Physical and biological sciences
影响因子：
--
作者：
Fusaki N;Ban H;Nishiyama A;Saeki K;Hasegawa M
通讯作者：
Hasegawa M
影响因子：
1.6
作者：
Nakamura, Naoko;Saeki, Kumiko;Yuo, Akira
通讯作者：
Yuo, Akira
影响因子：
14.8
作者：
Takahashi, Kazutoshi;Okita, Keisuke;Yamanaka, Shinya
通讯作者：
Yamanaka, Shinya