Oligofructose improves small intestinal lipid-sensing mechanisms via alterations to the small intestinal microbiota.
Oligofructose improves small intestinal lipid-sensing mechanisms via alterations to the small intestinal microbiota.
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DOI：
10.1186/s40168-023-01590-2
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发表时间：
2023-08-02
期刊：
影响因子：
15.5
通讯作者：
中科院分区：
文献类型：
--
作者：
上小肠膳食脂质激活肠-脑轴调节能量稳态。益生元低聚果糖（OFS）在代谢失调期间改善体重和肥胖，但确切机制仍不清楚。本研究探讨了OFS治疗后小肠微生物群的改变是否改善了小肠脂质感知，以调节高脂肪（HF）喂养大鼠的食物摄入。在喂食HF饮食4周的大鼠中，OFS补充剂在2天内减少了食物摄入量和膳食量，并在6周后减轻了体重和肥胖。急性（3天）OFS治疗恢复了HF喂养期间小肠脂质诱导的饱足感，并与小肠脂质输注后小肠CD 36表达、门静脉GLP-1水平和后脑神经元活化增加相关。将急性OFS治疗供体的小肠微生物群移植到HF喂养的大鼠中也恢复了脂质感应机制以降低食物摄入。16 S rRNA基因测序显示，长期和短期OFS都改变了小肠微生物群，增加了双歧杆菌的相对丰度。对HF喂养的大鼠小肠给予假长双歧杆菌改善小肠脂质敏感性以减少食物摄入。OFS补充剂快速调节小肠肠道微生物群，其介导控制食物摄入以改善能量稳态的小肠脂质传感机制的改善。视频摘要在线版本包含补充材料，可通过10. 1186/s40168-023-01590-2获取。
Upper small intestinal dietary lipids activate a gut-brain axis regulating energy homeostasis. The prebiotic, oligofructose (OFS) improves body weight and adiposity during metabolic dysregulation but the exact mechanisms remain unknown. This study examines whether alterations to the small intestinal microbiota following OFS treatment improve small intestinal lipid-sensing to regulate food intake in high fat (HF)-fed rats. In rats fed a HF diet for 4 weeks, OFS supplementation decreased food intake and meal size within 2 days, and reduced body weight and adiposity after 6 weeks. Acute (3 day) OFS treatment restored small intestinal lipid-induced satiation during HF-feeding, and was associated with increased small intestinal CD36 expression, portal GLP-1 levels and hindbrain neuronal activation following a small intestinal lipid infusion. Transplant of the small intestinal microbiota from acute OFS treated donors into HF-fed rats also restored lipid-sensing mechanisms to lower food intake. 16S rRNA gene sequencing revealed that both long and short-term OFS altered the small intestinal microbiota, increasing Bifidobacterium relative abundance. Small intestinal administration of Bifidobacterium pseudolongum to HF-fed rats improved small intestinal lipid-sensing to decrease food intake. OFS supplementation rapidly modulates the small intestinal gut microbiota, which mediates improvements in small intestinal lipid sensing mechanisms that control food intake to improve energy homeostasis. Video Abstract The online version contains supplementary material available at 10.1186/s40168-023-01590-2.
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DOI：
10.1038/ismej.2012.8
发表时间：
2012-08
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
--
影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
11.1
作者：
Amar, Jacques;Chabo, Chantal;Waget, Aurelie;Klopp, Pascale;Vachoux, Christelle;Bermudez-Humaran, Luis G.;Smirnova, Natalia;Berge, Mathieu;Sulpice, Thierry;Lahtinen, Sampo;Ouwehand, Arthur;Langella, Philippe;Rautonen, Nina;Sansonetti, Philippe J.;Burcelin, Remy
通讯作者：
Burcelin, Remy
影响因子：
4.9
作者：
Duca, F. A.;Swartz, T. D.;Covasa, M.
通讯作者：
Covasa, M.
影响因子：
24.5
作者：
Cani PD;Possemiers S;Van de Wiele T;Guiot Y;Everard A;Rottier O;Geurts L;Naslain D;Neyrinck A;Lambert DM;Muccioli GG;Delzenne NM
通讯作者：
Delzenne NM