Brain stimulation-on-a-chip: a neuromodulation platform for brain slices.
Brain stimulation-on-a-chip: a neuromodulation platform for brain slices.
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脑刺激芯片：脑切片的神经调节平台。
DOI：
10.1039/d3lc00492a
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发表时间：
2023-11-21
期刊：
影响因子：
6.1
通讯作者：
中科院分区：
文献类型：
--
作者：
离体脑组织切片的电刺激一直是用于理解经颅直流电刺激（tDCS）赋予的机制的方法，但是在常规实验设置中存在显著的直流电场（dcEF）剂量和电化学副产物问题，其可能影响转化结果。因此，我们开发了一种具有流体、电化学和磁诱导空间控制的片上平台。在流体方面，腔室在几何上将精确的dcEF递送限制在封闭的脑切片上，并允许组织恢复，以便监测刺激后效应。在电化学上，导电水凝胶电极减轻了常用金属电极典型的刺激诱导的法拉第反应。在磁性方面，我们在组织下方应用铁磁基质，并使用外部永磁体来实现与dcEF相关的原位旋转控制。通过将微流控室与活细胞钙成像和电生理记录相结合，我们展示了研究dcEF的急性和持久影响的潜力，并具有提供多会话刺激的潜力。这种片上生物电子平台提供了一种现代化但简单的解决方案，通过为用户提供更多的环境控制来电刺激受损组织，从而为进行彻底的脑刺激机制研究提供了新的机会。我们开发了一种微流体平台，用于对脑组织切片进行精确的电刺激。它克服了传统的电剂量和电化学问题，利用精确的流体，电化学和磁性控制。
Electrical stimulation of ex vivo brain tissue slices has been a method used to understand mechanisms imparted by transcranial direct current stimulation (tDCS), but there are significant direct current electric field (dcEF) dosage and electrochemical by-product concerns in conventional experimental setups that may impact translational findings. Therefore, we developed an on-chip platform with fluidic, electrochemical, and magnetically-induced spatial control. Fluidically, the chamber geometrically confines precise dcEF delivery to the enclosed brain slice and allows for tissue recovery in order to monitor post-stimulation effects. Electrochemically, conducting hydrogel electrodes mitigate stimulation-induced faradaic reactions typical of commonly-used metal electrodes. Magnetically, we applied ferromagnetic substrates beneath the tissue and used an external permanent magnet to enable in situ rotational control in relation to the dcEF. By combining the microfluidic chamber with live-cell calcium imaging and electrophysiological recordings, we showcased the potential to study the acute and lasting effects of dcEFs with the potential of providing multi-session stimulation. This on-chip bioelectronic platform presents a modernized yet simple solution to electrically stimulate explanted tissue by offering more environmental control to users, which unlocks new opportunities to conduct thorough brain stimulation mechanistic investigations. We developed a microfluidic platform for precise electrical stimulation on brain tissue slices. It overcomes conventional electric dosage and electrochemical concerns by utilizing precise fluidic, electrochemical, and magnetic control.
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