Chronic infection during placental malaria is associated with up-regulation of cycloxygenase-2.
Chronic infection during placental malaria is associated with up-regulation of cycloxygenase-2.
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DOI：
10.1186/1475-2875-9-45
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发表时间：
2010-02-09
期刊：
影响因子：
3
通讯作者：
Jambou R
中科院分区：
文献类型：
--
作者：
Sarr D;Aldebert D;Marrama L;Frealle E;Gaye A;Brahim HO;Niang M;Dangou JM;Mercereau-Puijalon O;Lehesran JY;Jambou R
胎盘性疟疾(PM)与胎儿发育不良有关，但其涉及的病理生理过程知之甚少。环氧合酶(COX)和脂氧合酶(LOX)将脂肪酸转化为前列腺素和白三烯，在妊娠和胎儿发育中起重要作用。目前针对特定药物的COX-2具有双重作用，因为它与子痫前期的病理和感染期间的恢复有关。通过量化两组感染和未感染疟疾的胎盘在分娩时COX-1、COX-2、15-LOX和IL-10的表达来质疑COX在PM中的作用。在分娩时收集胎盘活检组织，使用实时荧光定量聚合酶链式反应(Real Time PCR)进行mRNA分离和定量。COX-2和IL-10的mRNAs主要在慢性感染期间增加(分别是9倍和5倍)，而COX-1的转录本保持不变。COX-2的过度表达与婴儿出生体重较高有关，但与母亲的血红蛋白率较低有关。它与胎盘巨噬细胞的渗出和低的血球蛋白渗出有关。相反，胎盘感染与15-LOX mRNA的低表达有关。高度的血球蛋白沉积与低出生体重和环氧合酶-2表达减少相关。这些数据提供了证据，证明COX-2和IL-10在胎盘慢性感染期间高度诱导，但与早产或低出生体重无关。这些数据支持COX-2参与胎盘感染的恢复期。
Placental malaria (PM) is associated with poor foetal development, but the pathophysiological processes involved are poorly understood. Cyclooxygenase (COX) and lipoxygenase (LOX) which convert fatty acids to prostaglandins and leukotrienes, play important roles in pregnancy and foetal development. COX-2, currently targeted by specific drugs, plays a dual role as it associates with both pre-eclampsia pathology and recovery during infection. The role of COX during PM was questioned by quantifying at delivery COX-1, COX-2, 15-LOX, and IL-10 expression in two groups of malaria infected and uninfected placenta. Placental biopsies were collected at delivery for mRNA isolation and quantification, using real time PCR. COX-2 and IL-10 mRNAs increased mainly during chronic infections (nine- and five-times, respectively), whereas COX-1 transcripts remained constant. COX-2 over-expression was associated with a higher birth weight of the baby, but with a lower rate of haemoglobin of the mother. It was associated with a macrophage infiltration of the placenta and with a low haemozoin infiltration. In the opposite way, placental infection was associated with lower expression of 15-LOX mRNA. A high degree of haemozoin deposition correlates with low birth weight and decreased expression of COX-2. These data provide evidence that COX-2 and IL-10 are highly induced during chronic infection of the placenta, but were not associated with preterm delivery or low birth weight. The data support the involvement of COX-2 in the recovery phase of the placental infection.
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DOI：
10.1084/jem.20041836
发表时间：
2005-01-03
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
Meshnick, SR
影响因子：
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作者：
Lorenz, M;Slaughter, HS;Car, BD
通讯作者：
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