A collagen based cryogel bioscaffold coated with nanostructured polydopamine as a platform for mesenchymal stem cell therapy.
A collagen based cryogel bioscaffold coated with nanostructured polydopamine as a platform for mesenchymal stem cell therapy.
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DOI：
10.1002/jbm.a.36428
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发表时间：
2018-08
期刊：
影响因子：
--
通讯作者：
Thakor AS
中科院分区：
文献类型：
--
作者：
Razavi M;Hu S;Thakor AS
冷冻水凝胶（冷冻凝胶）是在零度以下温度下形成的聚合物水凝胶。由冷冻凝胶制成的生物支架具有相互连接的大孔，允许细胞迁移、组织向内生长、溶质不受阻碍的扩散和治疗剂的质量运输。在这项研究中，我们开发了基于胶原蛋白的冷冻凝胶生物支架，并使用简单的两步技术将其涂上聚多巴胺。 Cryogel 生物支架是在-20°C 下通过胶原交联合成的，并表现出具有 75% ± 63% 孔隙率的大孔互连结构。观察到两组孔径：直径300±50μm和30±10μm。使用成分分析证实了冷冻凝胶生物支架中添加了聚多巴胺涂层。这导致吸水率降低 41% ± 6 5%，溶胀率降低 81% ± 10%，溶解度降低 12% ± 3% (p < 0.05)，硬度增加 48% ± 2%，抗压强度增加 57% ± 5% (p < 0.05)。与接种到未包被的冷冻凝胶生物支架中的 ADMSC 相比，将脂肪组织来源的间充质干细胞 (AD-MSC) 接种到聚多巴胺涂层的冷冻凝胶生物支架中，细胞活力增加 52% ± 4%，增殖能力增加 33% ± 3% (p < 0.05)。总之，我们的新型聚多巴胺涂层冷冻凝胶生物支架代表了一种支持 MSC 治疗的高效且低成本的生物支架平台。
Cryo-hydrogels (cryogels) are polymer hydrogels formed at sub-zero temperatures. Bioscaffolds created from cryo-gels have interconnected macropores which allow for cell migration, tissue-ingrowth, unhindered diffusion of solutes and mass transport of therapeutics. In this study, we developed collagen based cryogel bioscaffolds and coated them with polydopamine using a simple two-step technique. Cryogel bioscaffolds were synthesized by collagen crosslinking at −20°C and exhibited a macroporous interconnected architecture with 75% ± 63% porosity. Two groups of pore sizes were observed: 300 ± 50 μm and 30 ± 10 μm in diameter. The addition of a polydopamine coating to cryogel bioscaffolds was confirmed using composition analysis. This resulted in a 41% ± 6 5% decrease in water uptake, 81% ± 10% decrease in swelling rate and 12% ± 3% decrease in their degree of dissolution (p < 0.05), with a 48% ± 2% increase in stiffness and 57% ± 5% increase in compressive strength (p < 0.05). Seeding adipose tissue-derived mesenchymal stem cells (AD-MSCs) into polydopamine coated-cryogel bioscaffolds resulted in cells demonstrating a 52% ± 4% increase in viability and 33% ±3% increase in proliferation when compared to ADMSCs seeded into uncoated-cryogel bioscaffolds (p < 0.05). In summary, our novel polydopamine coated-cryogel bioscaffold represents an efficient and low-cost bioscaffold platform to support MSC therapies.
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