High-density genetic map construction and mapping of the homologous transformation sterility gene (hts) in wheat using GBS markers
High-density genetic map construction and mapping of the homologous transformation sterility gene (hts) in wheat using GBS markers
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利用 GBS 标记构建小麦同源转化不育基因 (hts) 的高密度遗传图谱和定位
DOI：
10.1186/s12870-018-1532-x
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发表时间：
2018-11
影响因子：
5.3
通讯作者：
Zhengsong Peng
中科院分区：
文献类型：
--
作者：
Qian Yang;Zaijun Yang;Haifeng Tang;Yan Yu;Zhenyong Chen;Shuhong Wei;Qinxu Sun;Zhengsong Peng
背景同源转化不育-1（HTS-1）是一种新型小麦突变体，表现出雌蕊，即雄蕊转化为雌蕊或雌蕊样结构。这种突变的更极端的表型可以在小花中具有六个雌蕊或类似雌蕊的结构，而没有任何雄蕊。因此，HTS-1对于小麦杂交育种和花卉发育的研究具有很高的价值。先前的研究表明，两个主要基因（Pis1和hts）控制HTS-1的雌蕊。 Pis1基因控制三雌蕊小麦突变体的三雌蕊性状，并已被定位在2D染色体上，但hts基因尚未被定位或鉴定。为此，我们将 HTS-1 与 CM28TP（三雌蕊突变体）杂交，并构建了与 F2 群体（200 个个体）的高密度连锁图谱。结果该图谱覆盖 2779.96 cM，每条染色体的遗传距离范围为 37.59 cM 至 318.95 cM。标记之间的平均距离为1.04 cM。然后，我们在 GBS-SNP 标记 4A_109 和 4A_119 之间绘制图谱，间隔 2.0 cM 和 5.2 Mb。为了寻找候选基因，hts区域被扩大到7.2Mb，包含752个蛋白质编码基因。通过比较雌蕊雄蕊（PS）与正常雄蕊（S）以及雌蕊（P）与S之间的752个基因和206个常见差异表达基因，我们将TaWin1确定为可能的候选基因。实时PCR表明，TaWin1在雌蕊和雄蕊分化阶段在HTS-1中高表达，其水平比CM28TP高约120倍。进一步分析表明TaWin1主要在HTS-1 PS中表达，支持其作为hts候选基因的地位。因此，TaWin1的过表达可能导致小麦雄蕊向雌蕊的转化。结论本研究结果为进一步研究雄蕊和雌蕊的发育奠定了基础，对小麦杂交育种项目具有重要意义。
BackgroundHomologous transformation sterility-1 (HTS-1) is a novel wheat mutant that exhibits pistillody, the transformation of stamens into pistils or pistil-like structures. More extreme phenotypes of this mutation can have six pistils or pistil-like structures without any stamens in a floret. Thus, HTS-1 is highly valuable for studies of wheat hybrid breeding and flower development. Previous studies have shown that two major genes (Pis1andhts) control pistillody in HTS-1. ThePis1gene controls the three-pistil trait in the three-pistil wheat mutant and has been mapped on chromosome 2D, but thehtsgene has not been mapped or identified. To do so, we crossed HTS-1 with CM28TP (three-pistil mutant) and constructed a high-density linkage map with the F2population (200 individuals).ResultsThe map covered 2779.96 cM, and the genetic distance per chromosome ranged from 37.59 cM to 318.95 cM. The average distance between markers was 1.04 cM. We then mappedhtsbetween GBS-SNP markers 4A_109 and 4A_119, separated by 2.0 cM and 5.2 Mb. To find the candidate genes, thehtsregion was enlarged to 7.2 Mb, encompassing 752 protein-coding genes. We identifiedTaWin1as a possible candidate gene after comparing the 752 genes with 206 common differentially expressed genes between pistillody stamens (PS) versus normal stamens (S) and pistils (P) versus S. Real-time PCR indicated thatTaWin1was highly expressed in HTS-1 during the pistil-and-stamen-differentiating stage, at levels approximately 120 times greater than those in CM28TP. Further analysis indicated thatTaWin1was mainly expressed in HTS-1 PS, supporting its status as a candidate gene ofhts. Thus,TaWin1overexpression probably leads to the transformation of stamens into pistils in wheat.ConclusionsThe results of this study provide a foundation for further research on stamen and pistil development, with implications for wheat-hybrid breeding programs.
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影响因子：
9.2
作者：
Zimin AV;Puiu D;Hall R;Kingan S;Clavijo BJ;Salzberg SL
通讯作者：
Salzberg SL
利用特定位点扩增片段（SLAF）测序技术构建冬瓜果皮颜色高密度遗传图谱及基因定位
影响因子：
4.4
作者：
Jiang B;Liu W;Xie D;Peng Q;He X;Lin Y;Liang Z
通讯作者：
Liang Z
影响因子：
5.6
作者：
He J;Zhao X;Laroche A;Lu ZX;Liu H;Li Z
通讯作者：
Li Z
DOI：
10.2134/1999.geneticsandexploitation.c3
发表时间：
2015-11
期刊：
--
影响因子：
--
作者：
D. Duvick
通讯作者：
D. Duvick
异源四倍体棉花基因组组装：高密度分子遗传连锁图与 DNA 序列信息的整合
影响因子：
4.4
作者：
Zhao L;Yuanda L;Caiping C;Xiangchao T;Xiangdong C;Wei Z;Hao D;Xiuhua G;Wangzhen G
通讯作者：
Wangzhen G