GNS4, a novel allele of DWARF11, regulates grain number and grain size in a high-yield rice variety.
GNS4, a novel allele of DWARF11, regulates grain number and grain size in a high-yield rice variety.
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GNS4 是 DWARF11 的新型等位基因，可调节高产水稻品种的籽粒数量和籽粒大小
DOI：
10.1186/s12284-017-0171-4
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发表时间：
2017-12
期刊：
影响因子：
--
通讯作者：
Liang G
中科院分区：
文献类型：
--
作者：
Zhou Y;Tao Y;Zhu J;Miao J;Liu J;Liu Y;Yi C;Yang Z;Gong Z;Liang G
大米在食品安全方面发挥着极其重要的作用，因为它养活了世界上一半以上的人口。水稻籽粒产量取决于生物量和收获指数。突破水稻产量上限的一个重要策略是提高生物产量。因此，与器官大小相关的基因是水稻育种的重要目标。我们的特点是水稻突变体gns 4（4号染色体上的粒数和大小）与野生型相比，器官大小减少，每穗粒数减少，籽粒更小。图位克隆结果表明，GNS 4基因编码细胞色素P450蛋白，是DWARF 11（D11）的一个新等位基因。GNS 4启动子区的单核苷酸多态性（缺失）降低了其在突变体中的表达水平，导致谷粒数量减少和较小的谷粒。形态学和细胞分析表明，GNS 4正调节晶粒尺寸通过促进细胞伸长。在日本晴和武运粳7号（一个高产品种）背景下，过量表达GNS 4显著增加了器官大小、千粒重和穗大小，并随后提高了籽粒产量。这些结果表明，GNS 4基因是水稻产量育种中的关键目的基因，具有潜在的应用价值。 GNS 4被鉴定为水稻粒数和粒大小的正调控因子。在高产水稻品种中增加该基因的表达水平提高了籽粒产量。GNS 4可以在育种计划中作为目标，以提高产量。本文的在线版本（doi：10.1186/s12284-017-0171-4）包含补充材料，可供授权用户使用。
Rice plays an extremely important role in food safety because it feeds more than half of the world’s population. Rice grain yield depends on biomass and the harvest index. An important strategy to break through the rice grain yield ceiling is to increase the biological yield. Therefore, genes associated with organ size are important targets for rice breeding. We characterized a rice mutant gns4 (grain number and size on chromosome 4) with reduced organ size, fewer grains per panicle, and smaller grains compared with those of WT. Map-based cloning indicated that the GNS4 gene, encoding a cytochrome P450 protein, is a novel allele of DWARF11 (D11). A single nucleotide polymorphism (deletion) in the promoter region of GNS4 reduced its expression level in the mutant, leading to reduced grain number and smaller grains. Morphological and cellular analyses suggested that GNS4 positively regulates grain size by promoting cell elongation. Overexpression of GNS4 significantly increased organ size, 1000-grain weight, and panicle size, and subsequently enhanced grain yields in both the Nipponbare and Wuyunjing7 (a high-yielding cultivar) backgrounds. These results suggest that GNS4 is key target gene with possible applications in rice yield breeding. GNS4 was identified as a positive regulator of grain number and grain size in rice. Increasing the expression level of this gene in a high-yielding rice variety enhanced grain yield. GNS4 can be targeted in breeding programs to increase yields. The online version of this article (doi:10.1186/s12284-017-0171-4) contains supplementary material, which is available to authorized users.
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影响因子：
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作者：
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