The integration of 3-D cell printing and mesoscopic fluorescence molecular tomography of vascular constructs within thick hydrogel scaffolds.
The integration of 3-D cell printing and mesoscopic fluorescence molecular tomography of vascular constructs within thick hydrogel scaffolds.
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DOI：
10.1016/j.biomaterials.2012.04.004
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发表时间：
2012-07
期刊：
影响因子：
14
通讯作者：
Intes, Xavier
中科院分区：
文献类型：
--
作者：
Zhao, Lingling;Lee, Vivian K.;Yoo, Seung-Schik;Dai, Guohao;Intes, Xavier
关键词：
开发提供足够血管灌流的方法是构建大型功能组织工程的重要一步。同时，对于厚基质(>；2~3 mm)，缺乏评估血管通道的三维(3-D)结构和功能的成像方式。在此，我们报告了一种在厚水凝胶支架中构建和成像3-D动态灌流血管结构的原始方法。在这项工作中，我们将机器人三维细胞打印技术与中观荧光分子断层成像系统相结合，并展示了该平台能够构建具有内皮衬里的灌流胶原支架，并以高帧速率对流体流动和荧光标记的活内皮细胞进行成像，具有高灵敏度和准确性。这些结果为将3D细胞打印和荧光介观成像用于复杂组织工程组织的功能和分子研究奠定了基础。
Developing methods that provide adequate vascular perfusion is an important step toward engineering large functional tissues. Meanwhile, an imaging modality to assess the three-dimensional (3-D) structures and functions of the vascular channels is lacking for thick matrices (>2~3mm). Herein, we report on an original approach to construct and image 3-D dynamically perfused vascular structures in thick hydrogel scaffolds. In this work, we integrated a robotic 3-D cell-printing technology with a mesoscopic fluorescence molecular tomography imaging system, and demonstrated the capability of the platform to construct perfused collagen scaffolds with endothelial lining and to image both the fluid flow and fluorescent-labeled living endothelial cells at high-frame rates, with high sensitivity and accuracy. These results establish the potential of integrating both 3-D cell-printing and fluorescence mesoscopic imaging for functional and molecular studies in complex tissue engineered tissues.
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