Presence of pyrroloquinazoline alkaloid in Adhatoda vasica attenuates inflammatory response through the downregulation of pro-inflammatory mediators in LPS stimulated RAW 264.7 macrophages.
Presence of pyrroloquinazoline alkaloid in Adhatoda vasica attenuates inflammatory response through the downregulation of pro-inflammatory mediators in LPS stimulated RAW 264.7 macrophages.
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DOI：
10.34172/bi.2021.03
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Sujatha S
中科院分区：
文献类型：
--
作者：
Amala R;Sujatha S
前言：炎症是由有害刺激引起的主要反应，其次是血浆和免疫细胞从体内排入损伤组织部位的增加。一系列生长因子和细胞因子的信号级联在炎症部位传播并最终成熟，涉及损伤组织内的血管和免疫标志物，以促进退化组织的更新。在像糖尿病足溃疡这样的慢性疾病中，有一种顽固性炎症，它可能是截肢的主要因素，如果持续流行，甚至可能导致死亡。方法：采用Griess比色法检测了海参生物碱对RAW-264.7巨噬细胞产生一氧化氮的抑制作用，用ELISA法分析了肿瘤坏死因子-α和白细胞介素6(IL-6)的表达，用RT-α和Western blotting分析了环氧合酶-2和诱导型一氧化氮合酶的表达。用DCFH-DA探针法分析细胞内总ROS，用高效液相色谱(HPLC)证明ALK-F中含有喹唑啉生物碱(Vasicine)。结果：血管紧张素-F对脂多糖诱导的亚硝酸盐生成(13.2 ± 1.0 6µM)、诱导型一氧化氮合酶(2.6倍)和环氧合酶-2(3.3倍)均有明显的抑制作用，且呈剂量依赖关系。在最高浓度(1µg和10µg)的ALK-F作用下，促炎症细胞因子肿瘤坏死因子-α(1102 ± 1.02pg/mL)和IL-6(18 ± 0.87 ng/mL)的产生和细胞内总ROS均显著减少。用梯度洗脱法进行高效液相色谱分析，粗品生物碱中含有12%的喹唑啉生物碱。结论：因此，这项研究共同表明，一氧化氮的减少和炎症相关基因的失调，使血管紧张素有可能对抗炎症，并在糖尿病创面护理中提供治疗益处。
Introduction: Inflammation is the primary response caused due to harmful stimuli which are followed by the increased draining of plasma and immune cells from the body into the site of the injured tissue. A signaling cascade of growth factors and cytokines propagates and eventually matures in the inflammatory site involving the blood vessels and immune markers within the injured tissue in order to promote the renewal of the degenerated tissue. During a chronic disorder like diabetic foot ulcer, there is an obstinate inflammation which may act as a prime factor for limb amputation and upon persistent prevalence may even lead to death. Methods: This study focuses on the mode of action of ALK-F (alkaloid fraction) isolated from Adhatoda vasica in attenuating the nitric oxide production which was estimated by Griess assay, tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) expression was analyzed by ELISA and expression of COX-2 and iNOS by RT-PCR and western blotting in LPS stimulated RAW 264.7 macrophages. Total intracellular ROS was analyzed by DCFH-DA probing and the presence of quinazoline alkaloid (vasicine) in the ALK-F was evidenced by high performance liquid chromatography (HPLC). Results: The ALK-F of A. vasica exhibited a significant inhibitory effect on LPS elicited nitrite production (13.2 ± 1.06 µM), iNOS, and COX-2 (2.6 and 3.3 fold) in a dose-dependent manner. There was a significant decrease in the generation of these pro-inflammatory cytokines TNF-α (1102 ± 1.02 pg/mL) and IL-6 (18 ± 0.87 ng/mL) and total intracellular ROS in the highest tested concentrations (1 µg and 10 µg) of ALK-F of A. vasica. HPLC analysis by the gradient elution method revealed the presence of 12% of quinazoline alkaloid vasicine in the crude alkaloid fraction. Conclusion: Thus this study communally suggests that attenuation of nitric oxide and the dysregulation of genes responsible for inflammation which deliberates A. vasica to conflict against inflammation and provide remedial benefits in diabetic wound care.
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DOI：
10.1155/2014/625961
发表时间：
2014
期刊：
Evidence-based complementary and alternative medicine : eCAM
影响因子：
--
作者：
Nemudzivhadi V;Masoko P
通讯作者：
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影响因子：
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10.1161/atvbaha.110.207449
发表时间：
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影响因子：
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