In Vitro Assessment of Cytotoxicity, Antioxidant, and Anti-Inflammatory Activities of Ricinus communis (Euphorbiaceae) Leaf Extracts.
In Vitro Assessment of Cytotoxicity, Antioxidant, and Anti-Inflammatory Activities of Ricinus communis (Euphorbiaceae) Leaf Extracts.
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DOI：
10.1155/2014/625961
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Masoko P
中科院分区：
文献类型：
--
作者：
Nemudzivhadi V;Masoko P
蓖麻传统上被用作治疗炎症相关疾病的药物，包括伤口、溃疡和脓肿。以正己烷、二氯甲烷、丙酮、甲醇为萃取剂，采用逐级穷尽提取法对杜仲叶进行提取。采用ABTS+法定量测定各粗提物对2，2‘-叠氮基-双(3-乙基苯并噻唑啉-6-磺酸)自由基的抗氧化活性。以人白种皮肤成纤维细胞和巨噬细胞RAW-264.7细胞株为实验对象，研究了刺梨叶提取物的细胞毒作用和抗炎活性。甲醇提取物在2.5 mg/mLABTS+清除率最高，为95%，丙酮为91%，二氯甲烷为62%，正己烷最低(50%)。ABTS+自由基分子的清除活性百分率随着植物提取物浓度的增加而增加。正己烷和二氯甲烷提取物作用24小时和48小时后，在100 微克/毫升浓度下细胞存活率均在90%以上。甲醇提取物作用24小时的半数致死浓度为784 微克/毫升，正己烷为629.3 微克/毫升，二氯甲烷为573.6 微克/毫升，丙酮提取物最低为544.6 微克/毫升。本研究首次报道了杜仲叶提取物对ABTS+自由基的清除活性和对人皮肤成纤维细胞系的细胞毒作用。
Ricinus communis has been utilized traditionally as medicine to treat inflammatory related diseases including wounds, sores, and boils. The leaves of R. communis were sequentially extracted with n-hexane, dichloromethane, acetone, and methanol using serial exhaustive extraction method. Antioxidant activity of all crude extracts was quantitatively measured against 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) free radical molecules using ABTS+ assay. Cytotoxic effect and anti-inflammatory activity of R. communis leaves extracts were evaluated on Human Caucasian skin fibroblast and Raw 264.7 macrophage cell lines, respectively. Methanol extract had the highest percentage free radical (ABTS+) scavenging activity of 95% at 2.50 mg/mL, acetone 91%, dichloromethane 62%, and hexane the least (50%). Percentage scavenging activity of ABTS+ free radical molecules increases with increase in concentrations of the plant extracts. Hexane and dichloromethane extracts had more than 90% cell viability at 100 µg/mL after 24 and 48 hours of exposure. Methanol extract had LC50 of 784 µg/mL after 24-hour exposure, hexane had 629.3 µg/mL and dichloromethane 573.6 µg/mL, and 544.6 µg/mL was the lowest with acetone extract. The study present the first report on the scavenging activity of R. communis leaf extracts against ABTS+ radicals and cytotoxic effects on human Caucasian skin fibroblast cell lines.
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影响因子：
3.1
作者：
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通讯作者：
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DOI：
10.5402/2012/563267
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ISRN pharmacology
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