Neonatal ureteral obstruction stimulates recruitment of renin-secreting renal cortical cells.
Neonatal ureteral obstruction stimulates recruitment of renin-secreting renal cortical cells.
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新生儿输尿管梗阻会刺激分泌肾素的肾皮质细胞的募集。
DOI：
10.1038/ki.1994.174
复制
发表时间：
1994
影响因子：
19.6
通讯作者：
Chevalier,RL
中科院分区：
文献类型：
--
作者：
Norwood,VF;Carey,RM;Geary,KM;Jose,PA;Gomez,RA;Chevalier,RL
新生儿输尿管梗阻刺激分泌肾素的肾皮质细胞的募集。新生儿单侧输尿管梗阻（UUO）可增加同侧肾脏的肾素基因表达，这种作用是由肾神经介导的。为了确定新生儿UUO是否改变了分泌肾素的肾皮质细胞的数量，以及这种变化是否受到肾神经活动的调节，新生Sprague-Dawley大鼠进行左UUO，右单侧肾切除术，或假手术，并在4周后进行研究。为了评价肾神经在这种反应中的重要性，另一组动物接受了用乙啶进行的化学交感神经切除术。输尿管梗阻与梗阻肾的肾质量显著减少和对侧代偿性肥大相关，交感神经切除术不会改变这些变化。梗阻肾组织中的肾素信使RNA和肾组织中的肾素含量升高。通过反向溶血空斑试验测量的分泌肾素的细胞数量在梗阻肾中显著增加（45 ± 18个空斑/载玻片vs.假手术动物中11 ± 1个空斑/载玻片），但在对侧肾或单侧肾切除术后未增加。交感神经切除术没有显著改变这种效果。每个细胞分泌的肾素量或对Ca++的分泌反应没有变化。这些结果表明，UUO的结果在募集细胞以前不分泌肾素的机制独立于肾神经活动。这种募集的发生没有改变每个细胞分泌的肾素的量或Ca++对肾素分泌的正常调节作用。肾素分泌细胞数量的增加可能有助于肾素-血管紧张素系统的激活，从而导致输尿管梗阻后观察到的血管收缩。
Neonatal ureteral obstruction stimulates recruitment of renin-secreting renal cortical cells. Unilateral ureteral obstruction (UUO) in the neonate increases ipsilateral renal renin gene expression, an effect which is mediated by renal nerves. To determine whether neonatal UUO alters the number of renal cortical cells secreting renin and whether this change is modulated by renal nerve activity, newborn Sprague-Dawley rats were subjected to left UUO, right uninephrectomy, or sham operation and studied four weeks thereafter. To evaluate the importance of renal nerves in this response, an additional group of animals underwent chemical sympathectomy with guanethidine. Ureteral obstruction was associated with marked reduction in renal mass in the obstructed kidney and contralateral compensatory hypertrophy, changes which were not altered by sympathectomy. Renin messenger RNA and renal renin content were elevated in the obstructed kidney. The number of cells secreting renin, measured by the reverse hemolytic plaque assay, was markedly increased in the obstructed kidney (45 ± 18 plaques/slide vs. 11 ± 1 plaques/slide in sham animals), but not in the opposite kidney or following uninephrectomy. This effect was not significantly altered by sympathectomy. There was no change in the amount of renin secreted per cell or in the secretory response to Ca++. These results show that UUO results in recruitment of cells not previously secreting renin by a mechanism independent of renal nerve activity. This recruitment occurs without alteration of the quantity of renin secreted per cell or in the normal regulatory effect of Ca++on renin secretion. An increase in the number of renin-secreting cells may contribute to the activation of the renin-angiotensin system, and thus to the vasoconstriction observed following ureteral obstruction.
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DOI：
10.1172/jci115083
发表时间：
1991-03
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Samir S. El-Dahr;R. A. Gomez;Mark S. Gray;M. J. Peach;R. M. Carey;Robert L. Chevalier;M. Garmey-
通讯作者：
Samir S. El-Dahr;R. A. Gomez;Mark S. Gray;M. J. Peach;R. M. Carey;Robert L. Chevalier;M. Garmey-
影响因子：
--
作者：
G. Pfeifer;A. Riggs
通讯作者：
G. Pfeifer;A. Riggs
新生豚鼠慢性部分输尿管梗阻。
影响因子：
3.6
作者：
R. Chevalier;D. Kaiser
通讯作者：
D. Kaiser
实验性肾积水小鼠肾小球旁细胞的形态学和 Northern blot 分析
DOI：
--
发表时间：
1992
期刊：
The Anatomical Record
影响因子：
--
作者：
Y. Kon;S. Takahashi;A. Fukamizu;K. Murakami;Y. Hashimoto;M. Sugimura
通讯作者：
M. Sugimura
影响因子：
--
作者：
FROKIAER, J;KNUDSEN, L;DJURHUUS, JC
通讯作者：
DJURHUUS, JC