Genome sequence and functional genomic analysis of the oil-degrading bacterium Oleispira antarctica.
Genome sequence and functional genomic analysis of the oil-degrading bacterium Oleispira antarctica.
复制标题
DOI：
10.1038/ncomms3156
复制
发表时间：
2013
影响因子：
16.6
通讯作者：
Golyshin, Peter N.
中科院分区：
文献类型：
--
作者：
Kube, Michael;Chernikova, Tatyana N.;Al-Ramahi, Yamal;Beloqui, Ana;Lopez-Cortez, Nieves;Guazzaroni, Maria-Eugenia;Heipieper, Hermann J.;Klages, Sven;Kotsyurbenko, Oleg R.;Langer, Ines;Nechitaylo, Taras Y.;Luensdorf, Heinrich;Fernandez, Marisol;Juarez, Silvia;Ciordia, Sergio;Singer, Alexander;Kagan, Olga;Egorova, Olga;Petit, Pierre Alain;Stogios, Peter;Kim, Youngchang;Tchigvintsev, Anatoli;Flick, Robert;Denaro, Renata;Genovese, Maria;Albar, Juan P.;Reva, Oleg N.;Martinez-Gomariz, Montserrat;Tran, Hai;Ferrer, Manuel;Savchenko, Alexei;Yakunin, Alexander F.;Yakimov, Michail M.;Golyshina, Olga V.;Reinhardt, Richard;Golyshin, Peter N.
来自Oleispira属的无处不在的细菌在地球上最大的环境-寒冷和深海中推动石油降解。本文报道了南极油旋菌的基因组序列，并与嗜中温烃源细菌的范式-- anapartica有一个更大的基因组，见证了大规模的基因转移事件。我们确定了一系列的烷烃单加氧酶，植物保护剂，铁载体和微量营养素清除途径。我们还表明，在低温下，主要的蛋白质折叠机Cpn 60的功能作为一个单一的七聚体桶，使用较大的蛋白质作为基板相比，在较高的温度下观察到的经典双桶结构。11个蛋白质晶体结构，我们进一步报告了最大的一组结构从一个psychrotolerant生物。最常见的结构特征是与它们的嗜温对应物相比，表面暴露的带负电荷的残基的含量增加。我们的研究结果是相关的微生物冷适应机制的背景下，在寒冷环境中的石油泄漏缓解战略的发展。 海洋油旋菌是一种在寒冷深海中发现的石油降解细菌。Kube等人报道了O. anacritica，并提供全面的功能遗传和蛋白质结构分析，揭示了这种生物如何适应其寒冷环境的见解。
Ubiquitous bacteria from the genus Oleispira drive oil degradation in the largest environment on Earth, the cold and deep sea. Here we report the genome sequence of Oleispira antarctica and show that compared with Alcanivorax borkumensis—the paradigm of mesophilic hydrocarbonoclastic bacteria—O. antarctica has a larger genome that has witnessed massive gene-transfer events. We identify an array of alkane monooxygenases, osmoprotectants, siderophores and micronutrient-scavenging pathways. We also show that at low temperatures, the main protein-folding machine Cpn60 functions as a single heptameric barrel that uses larger proteins as substrates compared with the classical double-barrel structure observed at higher temperatures. With 11 protein crystal structures, we further report the largest set of structures from one psychrotolerant organism. The most common structural feature is an increased content of surface-exposed negatively charged residues compared to their mesophilic counterparts. Our findings are relevant in the context of microbial cold-adaptation mechanisms and the development of strategies for oil-spill mitigation in cold environments. Oleispira antarctica is an oil-degrading bacterium found in the cold and deep sea. Here Kube et al. report the genome sequence of O. antarctica and provide a comprehensive functional genetic and protein structural analysis, revealing insights into how this organism has adapted to its cold environment.
登录
查看更多内容
DOI：
10.1002/prot.10340
发表时间：
2003-06-01
期刊：
PROTEINS-STRUCTURE FUNCTION AND GENETICS
影响因子：
--
作者：
Kimber, MS;Vallee, F;Edwards, AM
通讯作者：
Edwards, AM
影响因子：
3.9
作者：
Lawrence, JG;Ochman, H
通讯作者：
Ochman, H
影响因子：
14.9
作者：
Lagesen K;Hallin P;Rødland EA;Staerfeldt HH;Rognes T;Ussery DW
通讯作者：
Ussery DW
影响因子：
4.4
作者：
Cavallaro, Gabriele;Decaria, Leonardo;Rosato, Antonio
通讯作者：
Rosato, Antonio
影响因子：
56.9
作者：
Camilli, Richard;Reddy, Christopher M.;Maloney, James V.
通讯作者：
Maloney, James V.