Molecular characterization of Giardia duodenalis and Cryptosporidium parvum in fecal samples of individuals in Mongolia.
Molecular characterization of Giardia duodenalis and Cryptosporidium parvum in fecal samples of individuals in Mongolia.
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DOI：
10.4269/ajtmh.13-0271
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发表时间：
2014-01
期刊：
影响因子：
--
通讯作者：
Lee SE
中科院分区：
文献类型：
--
作者：
Hong SH;Anu D;Jeong YI;Abmed D;Cho SH;Lee WJ;Lee SE
贾第鞭毛虫和隐孢子虫是广泛和频繁的寄生虫，影响人类和其他哺乳动物物种。目前尚不清楚这些寄生虫在蒙古的流行情况。因此，我们对G. tagalis和C.应用巢式聚合酶链反应（PCR）检测了蒙古国138例腹泻住院患者粪便标本中的细小病毒。分别有5份（3.62%）和7份（5.07%）粪便样本G. tagalis和C. parvum，分别。贾第鞭毛虫（Giardiacerialis）和C.细小病毒感染在9岁以下儿童中普遍存在。对G.经PCR-限制性片段多态性分析，5份阳性样品均属于A组。对7例阳性患者的18 S rDNA和HSP 70基因进行测序和系统发育分析，进一步鉴定为C.小型牛基因型。本研究首次报道了G. tagalis和C.蒙古腹泻个体粪便样本中的细小病毒及其分子特征。
The Giardia and Cryptosporidium species are widespread and frequent diarrhea-related parasites affecting humans and other mammalian species. The prevalence of these parasites in Mongolia is currently unknown. Therefore, we performed molecular analyses of G. duodenalis and C. parvum in stool samples from 138 patients hospitalized with diarrhea in Mongolia using nested polymerase chain reaction (PCR). A total of 5 (3.62%) and 7 (5.07%) fecal samples were positive for G. duodenalis and C. parvum, respectively. Giardia duodenalis and C. parvum infections were prevalent in children < 9 years of age. The assemblage-specific fragment patterns for the β-giardin gene of G. duodenalis revealed that all five samples testing positive belonged to Assemblage A by the PCR-restriction fragment polymorphism method. For sequencing and phylogenetic analysis of the 18S rDNA and HSP70 genes of all seven patients testing positive the genes were further identified to be of the C. parvum bovine genotype. This study is the first to report the prevalence of G. duodenalis and C. parvum and its molecular characterization of fecal samples from individuals with diarrhea in Mongolia.
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