Interactive effects of iron and light limitation on the molecular physiology of the Southern Ocean diatom Fragilariopsis kerguelensis
Interactive effects of iron and light limitation on the molecular physiology of the Southern Ocean diatom Fragilariopsis kerguelensis
复制标题
铁和光限制对南大洋硅藻 Fragilariopsisäkerguelensis 分子生理学的相互作用影响
DOI：
10.1002/lno.11404
复制
发表时间：
2020
影响因子：
4.5
通讯作者：
Marchetti, Adrian
中科院分区：
文献类型：
--
作者：
Moreno, Carly M.;Gong, Weida;Cohen, Natalie R.;DeLong, Kimberly;Marchetti, Adrian
极地硅藻Fragilariopsis kerguelenssis普遍存在于南大洋水域，是铁肥料的主要反应者，遇到铁浓度和光可获得性的大梯度。我们进行了一项比较转录分析。角胶在不同的铁和光条件下生长，以研究可能解释其对铁和光限制的生理反应的分子基础。低铁比弱光对生长速率的影响更大，但低铁和弱光对生长速率没有相加效应。低铁处理(饱和和弱光)的转录反应最大；然而，低光处理(高铁和低铁)的表达模式更相似。在铁和光照限制下，与对照(铁充足，饱和光照)相比，碳固定和氨基酸、核糖体和硫代谢被过度表达。在低铁/弱光处理中，水通道蛋白、蛋白视紫红质、叶绿体蓝蛋白和黄曲霉毒素等基因的转录水平最高，表明在分子水平上可能存在铁/光共生的加性效应。与限制生长的处理相比，铁和光充足的细胞表现出编码铁蛋白、碳酸氢酶和许多铁依赖蛋白的基因表达增加。这一转录组分析揭示了可能支持这种硅藻在低铁和光照环境中取得生态成功的机制，突出了各种光合作用异构体、铁获取、活性氧物种独特的解毒机制以及氨基酸循环和碳代谢中的代谢变化的重要作用。
The polar diatomFragilariopsis kerguelensisis ubiquitous in Southern Ocean waters and is a major responder to iron fertilization, encountering large gradients in iron concentrations and light availability. We performed a comparative transcriptomic analysis ofF. kerguelensisgrown under varying iron and light conditions in order to investigate the molecular underpinnings that may explain its physiological response to iron and light limitation. Low iron reduced growth rates more than low light, but there was not an additive effect of low iron and low light on growth rate. Low iron treatments (saturating and low light) had the largest transcriptomic response; however, expression patterns were more similar in low light treatments (high and low iron). Under iron and light limitation, carbon fixation and amino acid, ribosome, and sulfur metabolisms were overrepresented relative to the control (iron replete, saturating light). Transcripts of genes encoding for the proteins aquaporin, proteorhodopsin, plastocyanin, and flavodoxin were overrepresented to the greatest extent in the low iron/low light treatment, indicating there may be an additive effect of iron/light colimitation at the molecular level. Iron and light replete cells demonstrated increased expression of genes encoding for the proteins ferritin, carbonic anhydrase, and numerous iron‐dependent proteins relative to the growth‐limiting treatments. This transcriptome analysis reveals mechanisms that may underpin the ecological success of this diatom in low iron and light environments, highlighting the important role of diversified photosynthetic isoforms, iron acquisition, unique detoxification mechanisms of reactive oxygen species, and metabolic shifts in amino acid recycling and carbon metabolism.
登录
查看更多内容
影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
4.5
作者：
Koch, Florian;Beszteri, Sara;Trimborn, Scarlett
通讯作者：
Trimborn, Scarlett
影响因子：
2.9
作者：
Jochen Nuester;S. Vogt;B. Twining
通讯作者：
Jochen Nuester;S. Vogt;B. Twining
影响因子：
5.8
作者：
Simao, Felipe A.;Waterhouse, Robert M.;Zdobnov, Evgeny M.
通讯作者：
Zdobnov, Evgeny M.
影响因子：
13.6
作者：
Kazamia E;Sutak R;Paz-Yepes J;Dorrell RG;Vieira FRJ;Mach J;Morrissey J;Leon S;Lam F;Pelletier E;Camadro JM;Bowler C;Lesuisse E
通讯作者：
Lesuisse E