Spatiotemporal temperature distribution and cancer cell death in response to extracellular hyperthermia induced by gold nanorods.
Spatiotemporal temperature distribution and cancer cell death in response to extracellular hyperthermia induced by gold nanorods.
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DOI：
10.1021/nn901884d
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发表时间：
2010-05-25
期刊：
影响因子：
17.1
通讯作者：
Heys JJ
中科院分区：
文献类型：
--
作者：
Huang HC;Rege K;Heys JJ
无论是在体外还是在体内，等离子体纳米颗粒在热疗癌症治疗中都显示出了良好的前景。以前的报告已经描述了使用靶向和非靶向的纳米粒子在癌细胞内进行热疗，但大多数报告没有描述确定最佳参数的理论分析。目前的研究重点首先是评估细胞外高温对细胞的温度分布和细胞死亡的影响，其中金纳米棒(GNRs)分散在人前列腺癌细胞外。用近红外(NIR)激光照射纳米棒分散体，实验测定了纳米棒的温度时空分布。这些信息被用来开发和验证金纳米棒分散体在激光照射下的时空温度分布的理论模型，以及由此产生的热量对人类前列腺癌细胞活力的影响。然后将细胞损伤/死亡模型与热传递模型相耦合，以预测细胞死亡和损伤的时空变化。模型的预测与温度和细胞死亡分布的实验测量结果吻合得很好。最后，将该模型扩展到与非恶性细胞相比，考察了金纳米棒与癌细胞选择性结合的影响，以及细胞损伤激活能的微小变化。这些相对较小的变化的影响导致总体细胞死亡率的戏剧性变化。综上所述，使用金纳米棒进行细胞外热疗是一种很有前途的策略，而定制纳米棒的细胞结合效率可以导致使用这种方法的不同治疗效果。
Plasmonic nanoparticles have shown promise in hyperthermic cancer therapy, both in vitro and in vivo. Previous reports have described hyperthermic ablation using targeted and non-targeted nanoparticles internalized by cancer cells, but most reports do not describe a theoretical analysis for determining optimal parameters. The focus of the current research was first to evaluate the spatiotemporal temperature distribution and cell death induced by extracellular hyperthermia in which gold nanorods (GNRs) were maintained in the dispersion outside human prostate cancer cells. The nanorod dispersion was irradiated with near infrared (NIR) laser and the spatiotemporal distribution of temperature was determined experimentally. This information was employed to develop and validate theoretical models of spatiotemporal temperature profiles for gold nanorod dispersions undergoing laser irradiation, and the impact of the resulting heat generation on the viability of human prostate cancer cells. A cell injury/death model was then coupled to the heat transfer model to predict spatial and temporal variations in cell death and injury. The model predictions agreed well with experimental measurements of both, temperature and cell death profiles. Finally, the model was extended to examine the impact of selective binding of gold nanorods to cancer cells compared to non-malignant cells, coupled with a small change in cell injury activation energy. The impact of these relatively minor changes results in a dramatic change in the overall cell death rate. Taken together, extracellular hyperthermia using gold nanorods is a promising strategy and tailoring the cellular binding efficacy of nanorods can result in varying therapeutic efficacies using this approach.
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影响因子：
10.8
作者：
Durr, Nicholas J.;Larson, Timothy;Ben-Yakar, Adela
通讯作者：
Ben-Yakar, Adela
影响因子：
10.8
作者：
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El-Sayed, Mostafa A.
影响因子：
10.3
作者：
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通讯作者：
Chilkoti, A
影响因子：
3.8
作者：
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通讯作者：
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影响因子：
4.3
作者：
Dhar, Sheetal;Reddy, E. Maheswara;Prasad, B. L. V.
通讯作者：
Prasad, B. L. V.