Maltohexaose-indocyanine green (MH-ICG) for near infrared imaging of endocarditis.
Maltohexaose-indocyanine green (MH-ICG) for near infrared imaging of endocarditis.
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DOI：
10.1371/journal.pone.0247673
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发表时间：
2021
期刊：
影响因子：
3.7
通讯作者：
Taylor WR
中科院分区：
文献类型：
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作者：
Takemiya K;Røise JJ;He M;Taing C;Rodriguez AG;Murthy N;Goodman MM;Taylor WR
感染性心内膜炎是一种危及生命的疾病，迫切需要准确诊断这种疾病，尤其是人工瓣膜心内膜炎。我们在此表明，与吲哚菁绿缀合的麦芽六糖（MH-ICG）可以检测感染性心内膜炎大鼠模型中的金黄色葡萄球菌（S. aureus）感染。测定了 MH-ICG 对金黄色葡萄球菌的亲和力，其 Km 和 Vmax 分别为 5.4 μM 和 3.0 X 10−6 μmol/分钟/108 CFU。 MH-ICG 在浓度高达 100 μM 时对哺乳动物细胞没有可检测到的毒性。使用右心心内膜炎模型评估 MH-ICG 在大鼠体内的体内效率，细菌赘生物中 MH-ICG 的积累量比对照左心室壁高 2.5 ± 0.2 倍。 MH-ICG在健康大鼠中的生物半衰期为14.0±1.3分钟，24小时后大约50%注射的MH-ICG被排泄到粪便中。这些数据表明，MH-ICG 被细菌以高特异性内化，并且 MH-ICG 在心内膜炎大鼠模型的细菌赘生物中特异性积累。这些结果证明了该药物在检测感染性心内膜炎方面的潜在功效。
Infectious endocarditis is a life-threatening disease, and diagnostics are urgently needed to accurately diagnose this disease especially in the case of prosthetic valve endocarditis. We show here that maltohexaose conjugated to indocyanine green (MH-ICG) can detect Staphylococcus aureus (S. aureus) infection in a rat model of infective endocarditis. The affinity of MH-ICG to S. aureus was determined and had a Km and Vmax of 5.4 μM and 3.0 X 10−6 μmol/minutes/108 CFU, respectively. MH-ICG had no detectable toxicity to mammalian cells at concentrations as high as 100 μM. The in vivo efficiency of MH-ICG in rats was evaluated using a right heart endocarditis model, and the accumulation of MH-ICG in the bacterial vegetations was 2.5 ± 0.2 times higher than that in the control left ventricular wall. The biological half-life of MH-ICG in healthy rats was 14.0 ± 1.3 minutes, and approximately 50% of injected MH-ICG was excreted into the feces after 24 hours. These data demonstrate that MH-ICG was internalized by bacteria with high specificity and that MH-ICG specifically accumulated in bacterial vegetations in a rat model of endocarditis. These results demonstrate the potential efficacy of this agent in the detection of infective endocarditis.
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