Phylogenomics of the reproductive parasite Wolbachia pipientis wMel: a streamlined genome overrun by mobile genetic elements.
Phylogenomics of the reproductive parasite Wolbachia pipientis wMel: a streamlined genome overrun by mobile genetic elements.
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DOI：
10.1371/journal.pbio.0020069
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发表时间：
2004-03
期刊：
影响因子：
9.8
通讯作者：
Eisen JA
中科院分区：
文献类型：
--
作者：
Wu M;Sun LV;Vamathevan J;Riegler M;Deboy R;Brownlie JC;McGraw EA;Martin W;Esser C;Ahmadinejad N;Wiegand C;Madupu R;Beanan MJ;Brinkac LM;Daugherty SC;Durkin AS;Kolonay JF;Nelson WC;Mohamoud Y;Lee P;Berry K;Young MB;Utterback T;Weidman J;Nierman WC;Paulsen IT;Nelson KE;Tettelin H;O'Neill SL;Eisen JA
测定了黑腹果蝇专性胞内细菌Wolbachia limientis wMel的1,267,782bp基因组全序列。沃尔巴克氏菌广泛存在于各种无脊椎动物物种中，由于其与不同宿主的相互作用而引起人们的极大兴趣，这些宿主包括多种生殖寄生形式和互惠共生。对wMel基因组的分析，特别是与其他细胞内细菌的系统基因组比较，揭示了对wMel和Wolbachia的生物学和进化的许多见解。例如，wMEL基因组在已测序的专性细胞内物种中是独一无二的，因为它既高度简化，又包含非常高水平的重复DNA和可移动的DNA元件。这一观察，再加上多次进化重建，表明自然选择在wMel中有些低效，很可能是因为反复出现种群瓶颈。基因组分析预测了与立克次体密切相关的许多代谢差异，包括存在完整的糖酵解和嘌呤合成，这可能弥补了无法像立克次体那样直接从宿主获得ATP。其他发现包括wMel明显无法合成内毒素，以及存在大多数编码蛋白的基因，这些基因带有任何原核生物基因组中尚未测序的锚蛋白重复结构域。尽管wMel有能力感染其宿主的种系，但我们没有发现wMel和黑腹果蝇之间最近的侧向基因转移或沃尔巴克氏杆菌和任何宿主之间较老的转移的证据。进化分析进一步支持线粒体与α变形杆菌有共同祖先的假设，但几乎没有支持线粒体与立克次体菌目物种的分组。随着这两个物种的完整基因组的获得和宿主良好的遗传工具，wMEL-D黑腹共生现在是研究沃尔巴克氏菌感染生物学和进化的理想系统。沃尔巴克氏菌的基因组序列提供了对线粒体起源的洞察，以及内共生的生态和进化。
The complete sequence of the 1,267,782 bp genome of Wolbachia pipientis wMel, an obligate intracellular bacteria of Drosophila melanogaster, has been determined. Wolbachia, which are found in a variety of invertebrate species, are of great interest due to their diverse interactions with different hosts, which range from many forms of reproductive parasitism to mutualistic symbioses. Analysis of the wMel genome, in particular phylogenomic comparisons with other intracellular bacteria, has revealed many insights into the biology and evolution of wMel and Wolbachia in general. For example, the wMel genome is unique among sequenced obligate intracellular species in both being highly streamlined and containing very high levels of repetitive DNA and mobile DNA elements. This observation, coupled with multiple evolutionary reconstructions, suggests that natural selection is somewhat inefficient in wMel, most likely owing to the occurrence of repeated population bottlenecks. Genome analysis predicts many metabolic differences with the closely related Rickettsia species, including the presence of intact glycolysis and purine synthesis, which may compensate for an inability to obtain ATP directly from its host, as Rickettsia can. Other discoveries include the apparent inability of wMel to synthesize lipopolysaccharide and the presence of the most genes encoding proteins with ankyrin repeat domains of any prokaryotic genome yet sequenced. Despite the ability of wMel to infect the germline of its host, we find no evidence for either recent lateral gene transfer between wMel and D. melanogaster or older transfers between Wolbachia and any host. Evolutionary analysis further supports the hypothesis that mitochondria share a common ancestor with the α-Proteobacteria, but shows little support for the grouping of mitochondria with species in the order Rickettsiales. With the availability of the complete genomes of both species and excellent genetic tools for the host, the wMel–D. melanogaster symbiosis is now an ideal system for studying the biology and evolution of Wolbachia infections. The genome sequence of Wolbachia provides insights into the origins of mitochondria, as well as the ecology and evolution of endosymbiosis
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影响因子：
1.4
作者：
Eisen, JA;Wu, M
通讯作者：
Wu, M
影响因子：
30.8
作者：
Akman, L;Yamashita, A;Aksoy, S
通讯作者：
Aksoy, S
影响因子：
14.9
作者：
Eisen, JA
通讯作者：
Eisen, JA
DOI：
10.1098/rstb.2002.1193
发表时间：
2003-01-29
影响因子：
6.3
作者：
Andersson, SGE;Karlberg, O;Kurland, CG
通讯作者：
Kurland, CG
影响因子：
3.2
作者：
Braig, HR;Zhou, WG;O'Neill, SL
通讯作者：
O'Neill, SL