Coexistence of microplastics alters the inhibitory effect of antibiotics on sludge anaerobic digestion
Coexistence of microplastics alters the inhibitory effect of antibiotics on sludge anaerobic digestion
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微塑料的共存改变了抗生素对污泥厌氧消化的抑制作用
DOI：
10.1016/j.cej.2022.140754
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发表时间：
2022-12
影响因子：
15.1
通讯作者：
Dongbo Wang
中科院分区：
文献类型：
--
作者：
Yinping Xiang;Weiping Xiong;Zhaohui Yang;Rui Xu;Yanru Zhang;Mengru Wu;Yuhang Ye;Haihao Peng;Jing Tong;Dongbo Wang
微塑料和抗生素是新兴污染物，不可避免地进入污泥厌氧消化系统。目前的研究主要集中在微塑料或抗生素对甲烷产生的个体影响上。然而，它们之间也可能存在复杂的反应，并且它们对厌氧消化的综合影响仍不清楚。本研究通过批量和半连续试验揭示了聚酰胺和氧氟沙星的联合影响机制。短暂接触氧氟沙星不利于甲烷的产生。聚酰胺的共存能够部分吸附氧氟沙星，有效减轻低浓度氧氟沙星的抑制作用。聚酰胺还促进水解细菌和产酸剂（例如Hydrogenispora）的定植，并促进有机物转化为乙酸盐。但聚酰胺并不能有效缓解过量氧氟沙星对厌氧消化的抑制作用。高浓度的氧氟沙星刺激腐殖酸的释放，使甲烷产量减少80%以上，并将主要产甲烷途径从乙酰分解产甲烷转变为氢营养产甲烷。重组产酸和产甲烷途径，进一步探讨氧氟沙星对微生物代谢潜力的影响。宏基因组分析显示，在高浓度氧氟沙星长期胁迫下，大多数与糖酵解、氨基酸代谢和产甲烷相关的功能基因均下调。该研究阐明了氧氟沙星抑制有机物转化的微生物过程，揭示了聚酰胺和氧氟沙星对厌氧消化的联合影响，为评估多种污染物对污泥安全处置的影响提供了新的见解。
Microplastics and antibiotics are emerging pollutants that inevitably enter the sludge anaerobic digestion system. Current studies mainly focused on the individual effects of microplastics or antibiotics on methane generation. However, there may also be complex reactions between them and their combine effects on anaerobic digestion remain unclear. This study revealed the combined influencing mechanism of polyamide and ofloxacin through batch and semi-continuous tests. Brief exposure to ofloxacin was detrimental to methane production. The coexistence of polyamide enabled partial adsorption of ofloxacin, which effectively mitigates the inhibitory effect of ofloxacin at low concentrations. Polyamide also facilitated the colonization of hydrolytic bacteria and acidogens, such asHydrogenispora, and promoted the conversion of organic matter to acetate. However, polyamide could not effectively alleviate the inhibitory effect of excess ofloxacin on anaerobic digestion. Ofloxacin at high concentrations stimulated the release of humic acid, reduced methane production by more than 80%, and changed the predominant methanogenesis pathway from acetoclastic methanogenesis to hydrogenotrophic methanogenesis. Acidogenesis and methanogenesis pathways were restructured to further explore the effect of ofloxacin on the metabolic potentials of microorganisms. Metagenomic analysis revealed that most of functional genes associated with glycolysis, amino acid metabolism and methanogenesis were down regulated under long-term stress of high concentration of ofloxacin. This study elucidated the microbial process of organic matter conversion inhibition by ofloxacin, shed light on the combined effects of polyamide and ofloxacin on anaerobic digestion and provided new insights for assessing the impact of multiple pollutants on the safe disposal of sludge.
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影响因子：
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通讯作者：
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