Anti-Proliferation Activity of a Decapeptide from Perinereies aibuhitensis toward Human Lung Cancer H1299 Cells
Anti-Proliferation Activity of a Decapeptide from Perinereies aibuhitensis toward Human Lung Cancer H1299 Cells
复制标题
围沙蚕十肽对人肺癌H1299细胞的抗增殖活性
DOI：
10.3390/md17020122
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发表时间：
2019-02
期刊：
影响因子：
5.4
通讯作者：
Ding Guofang
中科院分区：
文献类型：
--
作者：
Jiang Shuoqi;Jia Yinglu;Tang Yunping;Zheng Die;Han Xingbiao;Yu Fangmiao;Chen Yan;Huang Fangfang;Yang Zuisu;Ding Guofang
沙蚕多肽(PAP)是从沙蚕酶解物中提纯的具有抗癌活性的十肽(Ile-Glu-Pro-Gly-Thr-Val-Gly-Met-Met-Phe，IEPGTVGMMF)。本研究观察了PAP对H1299细胞增殖的抑制作用。结果表明，PAP以时间和剂量依赖的方式促进H1299细胞凋亡，抑制H1299细胞增殖。当PAP浓度达到0.92 mM时，作用72 h后，95%以上的细胞死亡。倒置显微镜下观察细胞形态变化，AO/EB染色，流式细胞术检测细胞凋亡和细胞周期时相。结果表明，经PAP处理后，H1299细胞的早期和晚期凋亡率明显增加，总凋亡率明显高于对照组。此外，经PAP处理后，处于S期的细胞数量明显减少，细胞的增殖能力也降低。H1299细胞停滞于G2/M期，细胞周期进程受阻。Western blotting结果显示，nm23-H1和血管内皮生长因子(VEGF)蛋白水平呈剂量依赖性降低，而促凋亡蛋白和抗凋亡蛋白比值及凋亡相关caspase蛋白水平呈剂量依赖性升高。综上所述，我们的结果表明，PAP作为一种天然的海洋生物活性物质，对人肺癌H1299细胞具有抑制增殖和诱导凋亡的作用。PAP有可能被开发为功能性食品或佐剂，未来可能用于预防或治疗人类非小细胞肺癌。
Perinereis aibuhitensis peptide (PAP) is a decapeptide (Ile-Glu-Pro-Gly-Thr-Val-Gly-Met-Met-Phe, IEPGTVGMMF) with anticancer activity that was purified from an enzymatic hydrolysate of Perinereis aibuhitensis. In the present study, the anticancer effect of PAP on H1299 cell proliferation was investigated. Our results showed that PAP promoted apoptosis and inhibited the proliferation of H1299 cells in a time- and dose-dependent manner. When the PAP concentration reached 0.92 mM, more than 95% of treated cells died after 72 h of treatment. Changes in cell morphology were further analyzed using an inverted microscope and AO/EB staining and flow cytometry was adopted for detecting apoptosis and cell cycle phase. The results showed that the early and late apoptosis rates of H1299 cells increased significantly after treatment with PAP and the total apoptosis rate was significantly higher than that of the control group. Moreover, after treatment with PAP, the number of cells in the S phase of cells was significantly reduced and the ability for the cells to proliferate was also reduced. H1299 cells were arrested in the G2/M phase and cell cycle progression was inhibited. Furthermore, the results of western blotting showed that nm23-H1 and vascular endothelial growth factor (VEGF) protein levels decreased in a dose-dependent manner, while the pro-apoptotic protein and anti-apoptotic protein ratios and the level of apoptosis-related caspase protein increased in a dose-dependent manner. In conclusion, our results indicated that PAP, as a natural marine bioactive substance, inhibited proliferation and induced apoptosis of human lung cancer H1299 cells. PAP is likely to be exploited as the functional food or adjuvant that may be used for prevention or treatment of human non-small cell lung cancer in the future.
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影响因子：
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DOI：
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发表时间：
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期刊：
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影响因子：
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