Transcriptome profile analysis of young floral buds of fertile and sterile plants from the self-pollinated offspring of the hybrid between novel restorer line NR1 and Nsa CMS line in Brassica napus.
Transcriptome profile analysis of young floral buds of fertile and sterile plants from the self-pollinated offspring of the hybrid between novel restorer line NR1 and Nsa CMS line in Brassica napus.
复制标题
甘蓝型油菜新型恢复系 NR1 和 Nsa CMS 系杂交自花授粉后代的可育和不育植物幼花芽的转录组谱分析
DOI：
10.1186/1471-2164-14-26
复制
发表时间：
2013-01-16
期刊：
影响因子：
4.4
通讯作者：
Wei W
中科院分区：
文献类型：
--
作者：
Yan X;Dong C;Yu J;Liu W;Jiang C;Liu J;Hu Q;Fang X;Wei W
背景
Background
The fertile and sterile plants were derived from the self-pollinated offspring of the F1 hybrid between the novel restorer line NR1 and the Nsa CMS line in Brassica napus. To elucidate gene expression and regulation caused by the A and C subgenomes of B. napus, as well as the alien chromosome and cytoplasm from Sinapis arvensis during the development of young floral buds, we performed a genome-wide high-throughput transcriptomic sequencing for young floral buds of sterile and fertile plants.
Results
In this study, equal amounts of total RNAs taken from young floral buds of sterile and fertile plants were sequenced using the Illumina/Solexa platform. After filtered out low quality data, a total of 2,760,574 and 2,714,441 clean tags were remained in the two libraries, from which 242,163 (Ste) and 253,507 (Fer) distinct tags were obtained. All distinct sequencing tags were annotated using all possible CATG+17-nt sequences of the genome and transcriptome of Brassica rapa and those of Brassica oleracea as the reference sequences, respectively. In total, 3231 genes of B. rapa and 3371 genes of B. oleracea were detected with significant differential expression levels. GO and pathway-based analyses were performed to determine and further to understand the biological functions of those differentially expressed genes (DEGs). In addition, there were 1089 specially expressed unknown tags in Fer, which were neither mapped to B. oleracea nor to B. rapa, and these unique tags were presumed to arise basically from the added alien chromosome of S. arvensis. Fifteen genes were randomly selected and their expression levels were confirmed by quantitative RT-PCR, and fourteen of them showed consistent expression patterns with the digital gene expression (DGE) data.
Conclusions
A number of genes were differentially expressed between the young floral buds of sterile and fertile plants. Some of these genes may be candidates for future research on CMS in Nsa line, fertility restoration and improved agronomic traits in NR1 line. Further study of the unknown tags which were specifically expressed in Fer will help to explore desirable agronomic traits from wild species.
登录
查看更多内容
DOI：
10.3724/sp.j.1006.2008.01946
发表时间：
2008-01-01
期刊：
Acta Agronomica Sinica
影响因子：
--
作者：
Cheng JiHua, Cheng JiHua;Li YunChang, Li YunChang;Wang, W. M.
通讯作者：
Wang, W. M.
DOI：
10.1098/rstb.2002.1193
发表时间：
2003-01-29
影响因子：
6.3
作者：
Andersson, SGE;Karlberg, O;Kurland, CG
通讯作者：
Kurland, CG
影响因子：
11.6
作者：
Chen, WQ;Provart, NJ;Zhu, T
通讯作者：
Zhu, T
影响因子：
4.4
作者：
Hao QN;Zhou XA;Sha AH;Wang C;Zhou R;Chen SL
通讯作者：
Chen SL
影响因子：
11.6
作者：
Chen, JJ;Janssen, BJ;Sinha, N
通讯作者：
Sinha, N