Implication of the mosquito midgut microbiota in the defense against malaria parasites.
Implication of the mosquito midgut microbiota in the defense against malaria parasites.
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蚊子中肠菌群对疟疾寄生虫的防御作用。
DOI：
10.1371/journal.ppat.1000423
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发表时间：
2009-05
期刊：
影响因子：
6.7
通讯作者：
Dimopoulos G
中科院分区：
文献类型：
--
作者：
Dong Y;Manfredini F;Dimopoulos G
传播疟疾的蚊子持续暴露于微生物，包括它们的中肠微生物群。这种自然获得的微生物植物群可以通过一种未知的机制抑制疟原虫和其他人类病原体的发展来调节蚊子的媒介能力。我们已经进行了一个全面的功能基因组学的方法来阐明细菌的共同感染和人类恶性疟原虫在其天然载体冈比亚按蚊的发展之间的分子相互作用。脓毒症和无菌蚊子的全球转录谱确定了一个重要的免疫基因子集，这些基因主要由蚊子的微生物植物群上调，包括几种抗疟原虫因子。无微生物的无菌蚊子显示出对疟原虫感染的易感性增加，而用细菌和恶性疟原虫配子体共同喂养蚊子导致低于正常的感染水平。感染分析表明，细菌介导的抗疟原虫作用是由蚊子的抗微生物免疫应答介导的，可能是通过激活基础免疫。我们表明，微生物群可以调节一些免疫基因的抗疟原虫作用。总之，微生物群在调节蚊子维持疟原虫感染的能力方面起着至关重要的作用。冈比亚按蚊传播引起疟疾的寄生虫疟原虫，其肠道细菌植物群或微生物群包括多种物种。用抗生素治疗消除这种微生物群将使按蚊更容易感染疟原虫。在这项研究中，我们表明，这些细菌可以通过一种涉及蚊子免疫系统的机制来抑制蚊子感染人类疟疾寄生虫恶性疟原虫。我们的研究表明，蚊子的微生物植物群刺激了基础免疫活性，其中包括几个已知的抗疟原虫活性的因素。控制蚊子微生物群所需的相同免疫因子也可以防御疟疾寄生虫疟原虫。蚊子的微生物群，先天免疫系统和疟原虫寄生虫之间的这种复杂的相互作用可能对疟疾在该领域的传播具有重要意义，其中蚊子的细菌暴露可能在生态位之间存在很大差异。
Malaria-transmitting mosquitoes are continuously exposed to microbes, including their midgut microbiota. This naturally acquired microbial flora can modulate the mosquito's vectorial capacity by inhibiting the development of Plasmodium and other human pathogens through an unknown mechanism. We have undertaken a comprehensive functional genomic approach to elucidate the molecular interplay between the bacterial co-infection and the development of the human malaria parasite Plasmodium falciparum in its natural vector Anopheles gambiae. Global transcription profiling of septic and aseptic mosquitoes identified a significant subset of immune genes that were mostly up-regulated by the mosquito's microbial flora, including several anti-Plasmodium factors. Microbe-free aseptic mosquitoes displayed an increased susceptibility to Plasmodium infection while co-feeding mosquitoes with bacteria and P. falciparum gametocytes resulted in lower than normal infection levels. Infection analyses suggest the bacteria-mediated anti-Plasmodium effect is mediated by the mosquitoes' antimicrobial immune responses, plausibly through activation of basal immunity. We show that the microbiota can modulate the anti-Plasmodium effects of some immune genes. In sum, the microbiota plays an essential role in modulating the mosquito's capacity to sustain Plasmodium infection. The Anopheles gambiae mosquito that transmits the malaria-causing parasite Plasmodium has an intestinal bacterial flora, or microbiota, which comprises a variety of species. Elimination of this microbiota with antibiotic treatment will render the Anopheles mosquito more susceptible to Plasmodium infection. In this study we show that these bacteria can inhibit the infection of the mosquito with the human malaria parasite Plasmodium falciparum through a mechanism that involves the mosquito's immune system. Our study suggests that the microbial flora of mosquitoes is stimulating a basal immune activity, which comprises several factors with known anti-Plasmodium activity. The same immune factors that are needed to control the mosquito's microbiota are also defending against the malaria parasite Plasmodium. This complex interplay among the mosquito's microbiota, the innate immune system, and the Plasmodium parasite may have significant implications for the transmission of malaria in the field where the bacterial exposure of mosquitoes may differ greatly between ecological niches.
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影响因子：
9.8
作者：
Dong, Yuemei;Taylor, Harry E;Dimopoulos, George
通讯作者：
Dimopoulos, George
影响因子：
6.7
作者：
Dong Y;Aguilar R;Xi Z;Warr E;Mongin E;Dimopoulos G
通讯作者：
Dimopoulos G
影响因子：
32.4
作者：
Frolet, Cecile;Thoma, Martine;Levashina, Elena A.
通讯作者：
Levashina, Elena A.
影响因子：
2.1
作者：
BEIER, MS;PUMPUNI, CB;DAVIS, JR
通讯作者：
DAVIS, JR
DOI：
10.4269/ajtmh.1996.54.219
发表时间：
1996-02-01
影响因子：
3.3
作者：
DeMaio, J;Pumpuni, CB;Beier, JC
通讯作者：
Beier, JC