Development of a Reliable ic-ELISA with a Robust Antimatrix Interference Capability Based on QuEChERS Technology for the Rapid Detection of Zearalenone in Edible and Medical Coix Seeds and Subsequent Risk Assessments.
Development of a Reliable ic-ELISA with a Robust Antimatrix Interference Capability Based on QuEChERS Technology for the Rapid Detection of Zearalenone in Edible and Medical Coix Seeds and Subsequent Risk Assessments.
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基于 QuEChERS 技术开发具有强大抗基质干扰能力的可靠 ic-ELISA，用于快速检测食用和医用薏苡仁中的玉米赤霉烯酮并进行后续风险评估
DOI：
10.3390/foods11192983
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发表时间：
2022-09-24
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影响因子：
--
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中科院分区：
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间接竞争酶联免疫吸附测定（ic-ELISA）是大规模筛查检测霉菌毒素污染物的理想免疫测定方法。然而，在进行免疫分析时，复杂样品的基质效应一直是一个挑战，因为它会导致假阳性或阴性结果。本研究巧妙地采用便捷的QuEChERS技术结合优化稀释溶剂，消除了样品基质的干扰，大大提高了检测精度，并开发了可靠的ic-ELISAs，适用于60和500 μg/kg两个官方耐受水平，无需进一步校正即可筛查食用和药用薏苡仁中的玉米赤霉烯酮（ZEN）。对122批次薏苡仁进行检测，阳性率达97.54%。进一步分析了受污染的分布情况，随后对其食用和医用目的进行了风险评估。研究结果表明，食用 ZEN 污染水平较高的薏苡仁可能会对成年人的医疗和食用消费造成不良健康影响；即使在平均污染水平的情况下，与其他来源的薏苡仁用作食品时相比，ZEN对总风险的贡献更为突出；因此，有效的防控应该是未来的一个重要课题。
Indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) is an ideal immunoassay method for large-scale screenings to detect mycotoxin contaminants. However, the matrix effect of complicated samples has always been challenging when performing immunoassays, as it leads to false-positive or negative results. In this study, convenient QuEChERS technology combined with optimizing the dilution solvent was ingeniously used to eliminate interference from the sample matrix to greatly improve the detection accuracy, and reliable ic-ELISAs for the two official tolerance levels of 60 and 500 μg/kg were developed to screen zearalenone (ZEN) in edible and medical coix seeds without any further correction. Then, the 122 batches of coix seeds were determined, and the positive rate was up to 97.54%. The contaminated distribution was further analyzed, and risk assessment was subsequently performed for its edible and medical purposes. The findings indicated that consumption of coix seeds with higher ZEN contamination levels may cause adverse health effects for both medical and edible consumption in the adult population; even under the condition of average contamination level, ZEN from coix seeds was the more prominent contributor to the total risk compared to other sources when used as food; thus, effective prevention and control should be an essential topic in the future.
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