Gamma rays excited radioluminescence tomographic imaging.
Gamma rays excited radioluminescence tomographic imaging.
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伽马射线激发放射发光断层成像
DOI：
10.1186/s12938-018-0480-x
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发表时间：
2018-04-24
影响因子：
3.9
通讯作者：
Cao X
中科院分区：
文献类型：
--
作者：
Zhang X;Zhu S;Li Y;Zhan Y;Chen X;Kang F;Wang J;Cao X
放射性核素激发发光成像是利用临床核医学成像中放射性示踪剂衰变过程中产生的伽马射线等高能射线激发RLNPs发出的放射发光，揭示小动物体内放射发光纳米磷光体（RLNPs）分布的一种放射性核素光学成像策略。目前，还没有基于放射发光的层析成像的报道。方法提出了一种伽马射线激发放射发光层析成像（GRLT）方法，该方法通过对放射发光光子的表面测量数据进行逆算法重建图像，利用放射发光技术揭示小动物体内RLNPs的三维分布。利用扩散方程模拟了辐射发光光子在具有高散射和低吸收特性的生物组织中的传播。结果通过小鼠模型实验和人工源植入小鼠模型实验验证了GRLT的可行性，结果表明GRLT能够利用99mtc产生的伽马射线激发的放射发光信号揭示Gd2O2S:Tb等RLNPs的分布。结论随着靶向RLNPs的出现，GRLT可以为在细胞水平上进行生物过程的体内和无创检查提供新的可能性。特别是与切伦科夫发光成像相结合，GRLT可以利用单光学成像技术实现RLNPs和核素的双重分子信息。
BackgroundRadionuclide-excited luminescence imaging is an optical radionuclide imaging strategy to reveal the distributions of radioluminescent nanophosphors (RLNPs) inside small animals, which uses radioluminescence emitted from RLNPs when excited by high energy rays such as gamma rays generated during the decay of radiotracers used in clinical nuclear medicine imaging. Currently, there is no report of tomographic imaging based on radioluminescence.MethodsIn this paper, we proposed a gamma rays excited radioluminescence tomography (GRLT) to reveal three-dimensional distributions of RLNPs inside a small animal using radioluminescence through image reconstruction from surface measurements of radioluminescent photons using an inverse algorithm. The diffusion equation was employed to model propagations of radioluminescent photons in biological tissues with highly scattering and low absorption characteristics.ResultsPhantom and artificial source-implanted mouse model experiments were employed to test the feasibility of GRLT, and the results demonstrated that the ability of GRLT to reveal the distribution of RLNPs such as Gd2O2S:Tb using the radioluminescent signals when excited by gamma rays produced from99mTc.ConclusionsWith the emerging of targeted RLNPs, GRLT can provide new possibilities for in vivo and noninvasive examination of biological processes at cellular levels. Especially, combining with Cerenkov luminescence imaging, GRLT can achieve dual molecular information of RLNPs and nuclides using single optical imaging technology.
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影响因子：
3.6
作者：
Li C;Di K;Bec J;Cherry SR
通讯作者：
Cherry SR
体内纳米颗粒介导的放射性药物激发荧光分子成像
影响因子：
16.6
作者：
Hu, Zhenhua;Qu, Yawei;Wang, Kun;Zhang, Xiaojun;Zha, Jiali;Song, Tianming;Bao, Chengpeng;Liu, Haixiao;Wang, Zhongliang;Wang, Jing;Liu, Zhongyu;Liu, Haifeng;Tian, Jie
通讯作者：
Tian, Jie
影响因子：
3.8
作者：
Carpenter, C. M.;Sun, C.;Xing, L.
通讯作者：
Xing, L.
影响因子：
3.6
作者：
Pratx, Guillem;Carpenter, Colin M.;Xing, Lei
通讯作者：
Xing, Lei
影响因子：
3
作者：
Needell, Deanna;Vershynin, Roman
通讯作者：
Vershynin, Roman