Simultaneous measurement of neuronal and glial metabolism in rat brain in vivo using co-infusion of [1,6-13C2]glucose and [1,2-13C2]acetate.
Simultaneous measurement of neuronal and glial metabolism in rat brain in vivo using co-infusion of [1,6-13C2]glucose and [1,2-13C2]acetate.
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DOI：
10.1016/j.jmr.2008.11.001
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发表时间：
2009-02
期刊：
影响因子：
--
通讯作者：
Henry PG
中科院分区：
文献类型：
--
作者：
Deelchand DK;Nelson C;Shestov AA;Uğurbil K;Henry PG
本工作探讨了用[1，6-13C2]葡萄糖和[1，2-13C2]乙酸酯共输注测定大鼠脑内神经元-神经胶质代谢的可行性。在体内测量13C谱的时间进程，同时注入两种13C标记的底物。使用LC模型对单个13C同位素异构体(在13C谱中观察到的单态和多态)进行了自动定量。在谷氨酸和谷氨酰胺中观察到的独特的~(13)C谱型直接反映了这样一个事实，即葡萄糖主要在神经室代谢，乙酸盐主要在胶质室代谢。获得了谷氨酸和谷氨酰胺的单标记和多标记同位异构体的浓度时间曲线，时间分辨率为11min。尽管对这些13C同位素异构体数据的动态代谢建模将需要进一步的工作，这里没有报道，但我们预计这些新数据将允许更精确地确定代谢率，就像目前使用葡萄糖或醋酸酯作为唯一的13C标记底物时所可能的那样。
In this work the feasibility of measuring neuronal-glial metabolism in rat brain in vivo using co-infusion of [1,6-13C2]glucose and [1,2-13C2]acetate was investigated. Time courses of 13C spectra were measured in vivo while infusing both 13C-labeled substrates simultaneously. Individual 13C isotopomers (singlets and multiplets observed in 13C spectra) were quantified automatically using LCModel. The distinct 13C spectral pattern observed in glutamate and glutamine directly reflected the fact that glucose was metabolized primarily in the neuronal compartment and acetate in the glial compartment. Time courses of concentration of singly and multiply-labeled isotopomers of glutamate and glutamine were obtained with a temporal resolution of 11 min. Although dynamic metabolic modeling of these 13C isotopomer data will require further work and is not reported here, we expect that these new data will allow more precise determination of metabolic rates as is currently possible when using either glucose or acetate as the sole 13C-labeled substrate.
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影响因子：
7.4
作者：
de Graaf, RA;Behar, KL
通讯作者：
Behar, KL
影响因子：
4.2
作者：
Eloqayli, H;Dahl, CB;Sonnewald, U
通讯作者：
Sonnewald, U
影响因子：
6.3
作者：
Valette, Julien;Guillermier, Martine;Lebon, Vincent
通讯作者：
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影响因子：
5.3
作者：
Lebon, V;Petersen, KF;Rothman, DL
通讯作者：
Rothman, DL
DOI：
10.1073/pnas.0405065101
发表时间：
2004-08-24
影响因子：
11.1
作者：
de Graaf, RA;Mason, GF;Behar, KL
通讯作者：
Behar, KL