Vitamin D Deficiency Reduces Vascular Reactivity of Coronary Arterioles in Male Rats.
Vitamin D Deficiency Reduces Vascular Reactivity of Coronary Arterioles in Male Rats.
复制标题
DOI：
10.3390/cimb43010007
复制
发表时间：
2021-05-07
影响因子：
3.1
通讯作者：
Várbíró S
中科院分区：
文献类型：
--
作者：
Fontányi Z;Sziva RE;Pál É;Hadjadj L;Monori-Kiss A;Horváth EM;Benkő R;Magyar A;Heinzlmann A;Benyó Z;Nádasy GL;Masszi G;Várbíró S
背景：维生素D缺乏（VDD）可能被认为是一个独立的心血管（CV）危险因素，并且众所周知，男性的CV风险更高。我们的目的是研究不同维生素D供给情况下雄性大鼠冠状动脉壁段的药理学反应性和受体表达。方法：将4周龄雄性Wistar大鼠分为维生素D最佳供给量（300 IU/kgbw/ D）的对照组（n = 11）和VDD组（n = 11, <0.5 IU/kgbw/ D）。治疗8周后，制备冠状动脉壁内段，体外微血管测量法检测其药理反应性，免疫组化法检测其受体表达。结果：血栓素A2 （TXA2）激动剂诱导血管收缩减少，睾酮(T)和17-β-雌二醇（E2）松弛明显降低，血栓素受体（TP）表达明显降低，雌激素受体-α (ERα)表达明显降低。结论：vd缺乏的男性冠状动脉对TXA2和性类固醇的药理学反应性恶化（E2， T）。血管收缩能力不足伴TP受体表达降低，血管舒张损伤主要为功能性损伤。血管收缩剂和血管舒张剂反应的降低导致冠状动脉适应范围缩小，导致冠状动脉灌注不足，可能导致VDD患者心血管风险增加。
Background: Vitamin D deficiency (VDD) may be considered an independent cardiovascular (CV) risk factor, and it is well known that CV risk is higher in males. Our goal was to investigate the pharmacological reactivity and receptor expression of intramural coronary artery segments of male rats in cases of different vitamin D supply. Methods: Four-week-old male Wistar rats were divided into a control group (n = 11) with optimal vitamin D supply (300 IU/kgbw/day) and a VDD group (n = 11, <0.5 IU/kgbw/day). After 8 weeks of treatment, intramural coronary artery segments were microprepared, their pharmacological reactivity was examined by in vitro microangiometry, and their receptor expression was investigated by immunohistochemistry. Results: Thromboxane A2 (TXA2)-agonist induced reduced vasoconstriction, testosterone (T) and 17-β-estradiol (E2) relaxations were significantly decreased, a significant decrease in thromboxane receptor (TP) expression was shown, and the reduction in estrogen receptor-α (ERα) expression was on the border of significance in the VDD group. Conclusions: VD-deficient male coronary arteries showed deteriorated pharmacological reactivity to TXA2 and sexual steroids (E2, T). Insufficient vasoconstrictor capacity was accompanied by decreased TP receptor expression, and vasodilator impairments were mainly functional. The decrease in vasoconstrictor and vasodilator responses results in narrowed adaptational range of coronaries, causing inadequate coronary perfusion that might contribute to the increased CV risk in VDD.
登录
查看更多内容
影响因子：
20.3
作者：
Bouillon R;Marcocci C;Carmeliet G;Bikle D;White JH;Dawson-Hughes B;Lips P;Munns CF;Lazaretti-Castro M;Giustina A;Bilezikian J
通讯作者：
Bilezikian J
影响因子：
5.6
作者：
Braune S;Küpper JH;Jung F
通讯作者：
Jung F
影响因子：
4.6
作者：
Feng, Jun;Liu, Yuhong;Sellke, Frank W.
通讯作者：
Sellke, Frank W.
DOI：
10.1161/atvbaha.112.248039
发表时间：
2012-11-01
影响因子：
8.7
作者：
Brondum-Jacobsen, Peter;Benn, Marianne;Nordestgaard, Borge G.
通讯作者：
Nordestgaard, Borge G.
DOI：
10.1042/cs20170090
发表时间：
2017-07-01
期刊：
Clinical science (London, England : 1979)
影响因子：
--
作者：
Lucas-Herald AK;Alves-Lopes R;Montezano AC;Ahmed SF;Touyz RM
通讯作者：
Touyz RM