Autologous transplantation of oral mucosal epithelial cell sheets cultured on an amniotic membrane substrate for intraoral mucosal defects.
Autologous transplantation of oral mucosal epithelial cell sheets cultured on an amniotic membrane substrate for intraoral mucosal defects.
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DOI：
10.1371/journal.pone.0125391
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Kanamura N
中科院分区：
文献类型：
--
作者：
Amemiya T;Nakamura T;Yamamoto T;Kinoshita S;Kanamura N
人羊膜（AM）是一种薄的子宫内胎盘膜，具有高度的生物相容性，具有抗炎和抗疤痕的特性。利用AM技术，我们开发了一种培养口腔黏膜上皮细胞片的新方法。我们研究了AM培养的口腔黏膜上皮细胞在口腔手术患者中的自体移植。我们从剖宫产的妇女身上获得了AM标本。本研究包括5例没有任何内科病史的患者，他们在口腔外科手术后接受了自体培养的口腔上皮移植。利用这些患者的口腔黏膜活检标本，我们在AM载体上培养口腔上皮细胞。我们将生成的细胞片移植到口腔粘膜缺损上。患者在移植后至少随访12个月。在AM上培养2-3周后，上皮细胞分化良好，已成5 - 7层。免疫组化显示培养细胞表达高度特异性的粘膜上皮细胞标记物和基底膜蛋白。手术后，重建部位无感染、出血、排异反应或片状脱落，重建部位有明显的新口腔粘膜。长期随访无复发，术后病程良好。我们的研究结果表明，am培养的口腔黏膜上皮细胞片是一种有用的生物材料和可行的口腔粘膜重建方法。然而，我们的初步临床研究仅评估了它们对有限数量的小口腔粘膜缺陷的影响。
The human amniotic membrane (AM) is a thin intrauterine placental membrane that is highly biocompatible and possesses anti-inflammatory and anti-scarring properties. Using AM, we developed a novel method for cultivating oral mucosal epithelial cell sheets. We investigated the autologous transplantation of oral mucosal epithelial cells cultured on AM in patients undergoing oral surgeries. We obtained specimens of AM from women undergoing cesarean sections. This study included five patients without any history of a medical disorder who underwent autologous cultured oral epithelial transplantation following oral surgical procedures. Using oral mucosal biopsy specimens obtained from these patients, we cultured oral epithelial cells on an AM carrier. We transplanted the resultant cell sheets onto the oral mucosal defects. Patients were followed-up for at least 12 months after transplantation. After 2–3 weeks of being cultured on AM, epithelial cells were well differentiated and had stratified into five to seven layers. Immunohistochemistry revealed that the cultured cells expressed highly specific mucosal epithelial cell markers and basement membrane proteins. After the surgical procedures, no infection, bleeding, rejection, or sheet detachment occurred at the reconstructed sites, at which new oral mucous membranes were evident. No recurrence was observed in the long-term follow-up, and the postoperative course was excellent. Our results suggest that AM-cultured oral mucosal epithelial cell sheets represent a useful biomaterial and feasible method for oral mucosal reconstruction. However, our primary clinical study only evaluated their effects on a limited number of small oral mucosal defects.
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影响因子：
2
作者：
Koizumi, N;Inatomi, T;Kinoshita, S
通讯作者：
Kinoshita, S
影响因子：
4.6
作者：
Nishiyama, T;Amano, S;Burgeson, RE
通讯作者：
Burgeson, RE
影响因子：
4.1
作者：
Nakamura, T;Inatomi, T;Kinoshita, S
通讯作者：
Kinoshita, S
影响因子：
--
作者：
Black, AF;Bouez, C;Damour, O
通讯作者：
Damour, O
DOI：
10.1054/ijom.2002.0365
发表时间：
2003-04-01
影响因子：
2.4
作者：
Izumi, K;Feinberg, SE;Yoshizawa, M
通讯作者：
Yoshizawa, M