Differential Histopathological and Behavioral Outcomes Eight Weeks after Rat Spinal Cord Injury by Contusion, Dislocation, and Distraction Mechanisms.
Differential Histopathological and Behavioral Outcomes Eight Weeks after Rat Spinal Cord Injury by Contusion, Dislocation, and Distraction Mechanisms.
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DOI：
10.1089/neu.2015.4218
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发表时间：
2016-09-15
影响因子：
4.2
通讯作者：
Oxland TR
中科院分区：
文献类型：
--
作者：
Chen K;Liu J;Assinck P;Bhatnagar T;Streijger F;Zhu Q;Dvorak MF;Kwon BK;Tetzlaff W;Oxland TR
本研究的目的是比较三种不同的生物力学机制之一：脱位、挫伤和牵张导致的脊髓损伤(SCI)后的长期组织学和行为学结果。30只雄性SD大鼠被随机分为三种临床相关机制之一，建立创伤性颈髓损伤模型。对受伤的颈椎进行手术固定，并在接下来的8周内评估运动功能。然后取脊髓进行组织学分析。用Luxol快速蓝染色对白质的保留进行量化显示，脱位损伤导致了损伤脊髓外侧和沿吻尾轴的白质总体损失最大。牵张导致白质细胞外间隙扩大和结构改变，但总体上没有出现最多的有髓轴突。挫伤导致背侧白质中最严重的有髓轴突丢失。神经元标记物NeuN与荧光尼氏染色结合的免疫组织化学显示，错位机制导致腹角和背角的神经细胞丢失最多。在分心损伤机制后，与挫伤或脱位损伤的动物相比，随着时间的推移，动物的握力没有恢复。在错位损伤机制后，与挫伤或牵张损伤动物相比，动物在梳理测试中没有表现出改善。这些数据表明，不同的SCI机制导致不同的组织病理学和行为恢复模式。了解这种异质性对于未来针对脊髓损伤后特定神经病理的治疗干预措施的发展可能很重要。
The objective of this study was to compare the long-term histological and behavioral outcomes after spinal cord injury (SCI) induced by one of three distinct biomechanical mechanisms: dislocation, contusion, and distraction. Thirty male Sprague-Dawley rats were randomized to incur a traumatic cervical SCI by one of these three clinically relevant mechanisms. The injured cervical spines were surgically stabilized, and motor function was assessed for the following 8 weeks. The spinal cords were then harvested for histologic analysis. Quantification of white matter sparing using Luxol fast blue staining revealed that dislocation injury caused the greatest overall loss of white matter, both laterally and along the rostrocaudal axis of the injured cord. Distraction caused enlarged extracellular spaces and structural alteration in the white matter but spared the most myelinated axons overall. Contusion caused the most severe loss of myelinated axons in the dorsal white matter. Immunohistochemistry for the neuronal marker NeuN combined with Fluoro Nissl revealed that the dislocation mechanism resulted in the greatest neuronal cell losses in both the ventral and dorsal horns. After the distraction injury mechanism, animals displayed no recovery of grip strength over time, in contrast to the animals subjected to contusion or dislocation injuries. After the dislocation injury mechanism, animals displayed no improvement in the grooming test, in contrast to the animals subjected to contusion or distraction injuries. These data indicate that different SCI mechanisms result in distinct patterns of histopathology and behavioral recovery. Understanding this heterogeneity may be important for the future development of therapeutic interventions that target specific neuropathology after SCI.
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通讯作者：
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