Correlation between osteosarcoma and the expression of WWOX and p53.
Correlation between osteosarcoma and the expression of WWOX and p53.
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DOI：
10.3892/ol.2017.6747
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发表时间：
2017-10
期刊：
影响因子：
2.9
通讯作者：
Jin T
中科院分区：
文献类型：
--
作者：
Liu P;Wang M;Li L;Jin T
本研究旨在分析WWOX、p53表达对MG-63骨肉瘤细胞生长的影响，探讨骨肉瘤与WWOX、p53表达的相关性。通过转染建立WWOX和p53过表达的MG-63骨肉瘤细胞系，并分别命名为MW和MP细胞系。未转染的MG-63细胞（空白对照）用作对照。定量聚合酶链反应（qPCR）和蛋白质印迹分析分别检测WWOX和野生型p53 mRNA和蛋白的表达。通过MTT法和流式细胞术测定WWOX和p53（野生型）对MG-63细胞活性的影响。采用免疫组化方法检测65例骨肉瘤中突变型p53蛋白的表达，分析p53与骨肉瘤发生发展的相关性。 qPCR 显示 WWOX 和 p53 mRNA 分别在 MW 和 MP 细胞中过表达。 Western blot分析显示MW和MP细胞中WWOX和p53蛋白的水平高于空白对照组。 MTT法检测显示MW和MP细胞的细胞增殖能力明显低于空白对照组。流式细胞术显示78.49%的MW细胞和66.76%的MP细胞停滞在G0/G1期。免疫组化显示突变型p53在骨肉瘤中高表达，阳性表达率为47.7%。表达率与癌症的病理分级呈正相关。总之，WWOX可以影响MG-63骨肉瘤细胞的细胞周期，从而抑制细胞增殖，这为骨肉瘤的基因治疗提供了新的见解。两种类型的p53基因在骨肉瘤的发生发展中具有不同的功能。野生型p53作为肿瘤抑制因子，而突变型p53在恶性骨肉瘤中过度表达，具有与骨肉瘤程度相关的致癌作用。
The objective of this study was to analyze the effect of the expression of WWOX and p53 on the growth of MG-63 osteosarcoma cells and to explore the correlation between osteosarcoma and the expression of WWOX and p53. WWOX and p53-overexpressing MG-63 osteosarcoma cell lines were established by transfection and named the MW and MP cell lines, respectively. Untransfected MG-63 cells (blank control) were used as control. Quantitative polymerase chain reaction (qPCR) and western blot analysis were used to detect the expression of WWOX and wild-type p53 mRNA and protein, respectively. The effects of WWOX and p53 (wild-type) on the activity of MG-63 cells were determined by MTT assay and flow cytometry. The expression of mutant p53 protein in 65 cases of osteosarcoma was detected by immunohistochemistry to analyze the correlation between p53 and the development of osteosarcoma. qPCR showed that WWOX and p53 mRNA was overexpressed in MW and MP cells, respectively. Western blot analysis showed that the levels of WWOX and p53 protein in MW and MP cells were higher than in the blank control group. MTT assay showed that the cell proliferation ability of MW and MP cells was significantly lower than in the blank control group. Flow cytometry showed that 78.49% of MW and 66.76% of MP cells were arrested in the G0/G1 phase. Immunohistochemistry showed that mutant p53 was highly expressed in osteosarcoma, with a positive expression rate of 47.7%. The expression rate was positively correlated with the pathological grade of cancer. In conclusion, WWOX can affect the cell cycle of MG-63 osteosarcoma cells to inhibit cell proliferation, which provides new insights into gene therapy for osteosarcoma. The two types of the p53 gene have different functions in the development of osteosarcoma. Wild-type p53 acts as a tumor suppressor, while mutant p53, which is overexpressed in malignant osteosarcoma, has a carcinogenic effect associated with the degree of osteosarcoma.
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影响因子：
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作者：
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