A biomimetic hyaluronic acid-silk fibroin nanofiber scaffold promoting regeneration of transected urothelium.
A biomimetic hyaluronic acid-silk fibroin nanofiber scaffold promoting regeneration of transected urothelium.
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一种促进横断尿路上皮再生的仿生透明质酸-丝素纳米纤维支架。
DOI：
10.1002/btm2.10268
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发表时间：
2022-05
影响因子：
7.4
通讯作者：
Xia, Huimin
中科院分区：
文献类型：
--
作者：
Niu, Yuqing;Galluzzi, Massimiliano;Deng, Fuming;Zhao, Zhang;Fu, Ming;Su, Liang;Sun, Weitang;Jia, Wei;Xia, Huimin
本研究旨在探讨透明质酸（HA）涂层丝素蛋白（SF）纳米纤维在尿道上皮化过程中对尿道再生的调节作用。所获得的电纺仿生管状 HA-SF 纳米纤维支架由致密内层和多孔外层组成，分别模拟天然组织的粘附层和海绵层。在内壁固定一层薄薄的HA凝胶涂层，使SF纳米纤维具有致密光滑的表面纳米形貌和更高的亲水性。与纯SF纳米纤维相比，HA-SF纳米纤维显着促进原代尿路上皮细胞的粘附、生长和增殖，并上调uroplakin-3（尿路上皮终末分化角蛋白）的表达。利用新西兰雄性兔尿道损伤模型，由管状HA-SF纳米纤维组成的支架可以从宿主邻近区域招募管腔和肌上皮细胞，快速重建伤口区域的尿路上皮屏障，以保持尿道通畅，从而促进管腔上皮化、平滑肌束结构重塑和毛细血管形成。总体而言，纳米形貌和生物物理线索在仿生支架设计中的协同作用可实现有效的内源性再生。
This study was designed to investigate the regulatory effect of hyaluronic acid (HA)—coating silk fibroin (SF) nanofibers during epithelialization of urinary tract for urethral regeneration. The obtained electrospun biomimetic tubular HA‐SF nanofiber scaffold is composed of a dense inner layer and a porous outer layer in order to mimic adhesion and cavernous layers of the native tissue, respectively. A thin layer of HA‐gel coating was fixed in the inner wall to provide SF nanofibers with a dense and smooth surface nano‐topography and higher hydrophilicity. Compared with pure SF nanofibers, HA‐SF nanofibers significantly promoted the adhesion, growth, and proliferation of primary urothelial cells, and up‐regulate the expression of uroplakin‐3 (terminal differentiation keratin protein in urothelium). Using the New Zealand male rabbit urethral injury model, the scaffold composed of tubular HA‐SF nanofibers could recruit lumen and myoepithelial cells from the adjacent area of the host, rapidly reconstructing the urothelial barrier in the wound area in order to keep the urinary tract unobstructed, thereby promoting luminal epithelialization, smooth muscle bundle structural remodeling, and capillary formation. Overall, the synergistic effects of nano‐topography and biophysical cues in a biomimetic scaffold design for effective endogenous regeneration.
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