Monitoring IgG against Mycobacterium tuberculosis proteins in an Asian elephant cured of tuberculosis that developed from long-term latency.
Monitoring IgG against Mycobacterium tuberculosis proteins in an Asian elephant cured of tuberculosis that developed from long-term latency.
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DOI：
10.1038/s41598-022-08228-7
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发表时间：
2022-03-12
影响因子：
4.6
通讯作者：
Matsumoto S
中科院分区：
文献类型：
--
作者：
Ishikawa S;Ozeki Y;Suga S;Mukai Y;Kobayashi H;Inouchi E;Kaboso SA;Gebretsadik G;Dewi DNSS;Nishiyama A;Tateishi Y;Takihara H;Okuda S;Yoshida S;Misawa N;Matsumoto S
结核病（TB）对大象来说是致命的，因此保护大象免受结核病的侵害不仅是保护这种濒危动物的关键，也是防止结核病从大象传播给人类的关键。大多数人类结核病病例是由长期无症状感染引起的。由于大象身体庞大，在检测大象潜伏期的感染和疾病发展方面仍然存在重大的诊断挑战。在这项研究中，我们评估了从日本首例大象结核病治疗病例中收集了 16 年多的冷冻血清。对 11 种蛋白质的 IgG 水平进行半定量，结果显示 3 种蛋白质的检测水平较高，即 ESAT6/CFP10、MPB83 和 Ag85B。对这 3 种抗原特异性的 IgG 水平进行了纵向测量，结果显示无论发病或治疗如何，ESAT6/CFP10 IgG 水平都较高且稳定。 Ag85B 特异性 IgG 水平在很大程度上对发病或治疗有反应，而 MPB83 的 IgG 水平则表现出中等反应。这些结果表明，ESAT6/CFP10 在无症状和有症状阶段均具有免疫优势，使其可用于检测感染。另一方面，Ag85B 有潜力成为预测大象疾病发作和评估治疗效果的标记。
Tuberculosis (TB) is fatal in elephants, hence protecting elephants from TB is key not only in the conservation of this endangered animal, but also to prevent TB transmission from elephants to humans. Most human TB cases arise from long-term asymptomatic infections. Significant diagnostic challenges remain in the detection of both infection and disease development from latency in elephants due to their huge bodies. In this study, we assessed cryopreserved sera collected for over 16 years, from the first Japanese treatment case of elephant TB. Semi-quantification of IgG levels to 11 proteins showed high detection levels of 3 proteins, namely ESAT6/CFP10, MPB83 and Ag85B. The level of IgG specific to these 3 antigens was measured longitudinally, revealing high and stable ESAT6/CFP10 IgG levels regardless of onset or treatment. Ag85B-specifc IgG levels were largely responsive to onset or treatment, while those of MPB83 showed intermediate responses. These results suggest that ESAT6/CFP10 is immunodominant in both asymptomatic and symptomatic phases, making it useful in the detection of infection. On the other hand, Ag85B has the potential to be a marker for the prediction of disease onset and in the evaluation of treatment effectiveness in elephants.
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影响因子：
4.6
作者：
Inoue M;Niki M;Ozeki Y;Nagi S;Chadeka EA;Yamaguchi T;Osada-Oka M;Ono K;Oda T;Mwende F;Kaneko Y;Matsumoto M;Kaneko S;Ichinose Y;Njenga SM;Hamano S;Matsumoto S
通讯作者：
Matsumoto S
影响因子：
--
作者：
Backues, K. A.;Wiedner, E. B.
通讯作者：
Wiedner, E. B.
影响因子：
0.7
作者：
Mikota, SK;Peddie, L;Maslow, J
通讯作者：
Maslow, J
影响因子：
4.5
作者：
Lee, Ji Yeon;Kim, Byoung-Jun;Kim, Bum-Joon
通讯作者：
Kim, Bum-Joon
影响因子：
6.4
作者：
Kunnath-Velayudhan, Shajo;Davidow, Amy L.;Gennaro, Maria L.
通讯作者：
Gennaro, Maria L.