The differentiation of preadipocytes and gene expression related to adipogenesis in ducks (Anas platyrhynchos).
The differentiation of preadipocytes and gene expression related to adipogenesis in ducks (Anas platyrhynchos).
复制标题
鸭（Anas platyrhynchos）前脂肪细胞的分化和与脂肪形成相关的基因表达
DOI：
10.1371/journal.pone.0196371
复制
发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Chen G
中科院分区：
文献类型：
--
作者：
Wang S;Zhang Y;Xu Q;Yuan X;Dai W;Shen X;Wang Z;Chang G;Wang Z;Chen G
鸭的肉质与脂肪组织密切相关，脂肪的形成是由一个复杂的转录因子网络控制的，这些转录因子紧密地作用于鸭的不同分化阶段。本研究旨在建立鸭前脂肪细胞体外培养体系，从细胞和分子水平了解鸭脂肪细胞组织扩增的生物学特性。从3个品种鸭的皮下脂肪中分离前脂肪细胞，用胰岛素、罗格列酮、地塞米松、3-异丁基-1-甲基黄嘌呤和油酸的混合物分别诱导其分化为成熟脂肪细胞，培养时间分别为0、2、4、6和8 d。从脂滴的发育及其对油红O的反应证实了成功的分化，并且随着时间的推移，越来越多的脂滴被染成红色。在前脂肪细胞分化过程中，过氧化物酶体增殖物激活受体γ（PPARγ）、CCAAT/增强子结合蛋白-α（C/EBPα）、脂肪细胞脂肪酸结合蛋白4（FABP 4）和脂肪酸合成酶（FAS）等关键标志基因的表达逐渐增加。干扰实验进一步证实，抑制PPARγ表达可直接降低脂质的生成。同时分析了不饱和脂肪酸在不同浓度油酸制备家禽脂肪中的作用，发现油酸浓度为300 μM时，脂肪滴沉积量最高。本研究还比较了建昌鸭（肉鸭）、樱桃谷鸭（瘦肉鸭）和白冠鸭（蛋鸭）的分化前脂肪细胞水平。建昌鸭前脂肪细胞的增殖分化率高于白冠鸭。这些结果为进一步研究水禽脂肪形成提供了基础。
Meat quality is closely related to adipose tissues in ducks, and adipogenesis is controlled by a complex network of transcription factors tightly acting at different stages of differentiation especially in ducks. The aim of this study was to establish the preadipocyte in vitro culture system and understand the biological characteristics of expansion of duck adipocyte tissue at the cellular and molecular level. We isolated pre-adipocytes from the subcutaneous fat of three breeds of duck and differentiated them into mature adipocytes using a mixture of insulin, rosiglitazone, dexamethasone, 3-isobutyl-1-methylxanthine, and oleic acid over 0,2, 4, 6, and 8 days. Successful differentiation was confirmed from the development of lipid droplets and their response to Oil Red O, and increasing numbers of lipid droplets were stained red over time. The expression of key marker genes, including peroxisome proliferator activated receptor γ (PPARγ), CCAAT/enhancer binding protein-α (C/EBPα), adipocyte fatty acid binding protein 4 (FABP4), and fatty acid synthetase (FAS), gradually increased during pre-adipocyte differentiation. Furthermore, it was verified by interference experiments that the knockdown of PPARγ directly reduced lipid production. Meanwhile we analyzed the role of unsaturated fatty acids in the production of poultry fat using different concentrations of oleic acid and found that lipid droplet deposition was highest when the concentration of oleic acid was 300 μM. We also compared the level of differentiated pre-adipocytes that were isolated from Jianchang ducks (fatty-meat duck), Cherry Valley ducks (lean-meat duck) and White-crested ducks (egg-producing duck). The proliferation and differentiation rate of pre-adipocytes derived from Jianchang ducks was higher than that of White-crested ducks. These results provide the foundation for further research into waterfowl adipogenesis.
登录
查看更多内容
影响因子：
2.6
作者：
Covas, Dimas T.;Panepucci, Rodrigo A.;Zago, Marco A.
通讯作者：
Zago, Marco A.
影响因子：
4.9
作者：
Farmer, SR
通讯作者：
Farmer, SR
影响因子：
10.5
作者：
Rosen, ED;Hsu, CH;Spiegelman, BM
通讯作者：
Spiegelman, BM
影响因子：
1.2
作者：
Mitterberger, Maria C.;Lechner, Stefan;Zwerschke, Werner
通讯作者：
Zwerschke, Werner
影响因子：
7.1
作者：
Yamada, T.;Kawakami, S. -I;Nakanishi, N.
通讯作者：
Nakanishi, N.