Effects of prophylactic administration of bacteriophages to immunosuppressed mice infected with Staphylococcus aureus.
Effects of prophylactic administration of bacteriophages to immunosuppressed mice infected with Staphylococcus aureus.
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DOI：
10.1186/1471-2180-9-169
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发表时间：
2009-08-17
期刊：
影响因子：
4.2
通讯作者：
Górski A
中科院分区：
文献类型：
--
作者：
Zimecki M;Artym J;Kocieba M;Weber-Dabrowska B;Borysowski J;Górski A
噬菌体可以成功地用于治疗由耐抗生素细菌引起的感染。到目前为止，还没有人尝试用噬菌体治疗免疫抑制患者的感染。本研究探讨了特异性噬菌体对环磷酰胺（CP）处理和金黄色葡萄球菌感染的CBA小鼠的预防作用。应用噬菌体后，cp处理和金黄色葡萄球菌感染小鼠的器官中大量细菌集落形成单位以及升高的肿瘤坏死因子和白细胞介素-6血清浓度均显著降低。在cp处理的金黄色葡萄球菌感染的小鼠中，噬菌体显著增加了循环中性粒细胞和来自髓细胞和淋巴细胞谱系的未成熟细胞的百分比，以及骨髓中髓细胞和未成熟中性粒细胞的百分比。此外，噬菌体刺激这些小鼠产生特异性的抗金黄色葡萄球菌凝集素。在金黄色葡萄球菌感染前对免疫抑制小鼠应用特异性噬菌体证明非常有效，这表明噬菌体治疗对经历细菌感染的免疫功能低下患者有潜在的益处。
Bacteriophages can be successfully applied to treat infections caused by antibiotic-resistant bacteria. Until now no attempts have been undertaken to treat infections in immunosuppressed patients with phages. In this work we investigated the prophylactic efficacy of specific bacteriophages in CBA mice treated with cyclophosphamide (CP) and infected with Staphylococcus aureus. High numbers of bacterial colony-forming units in the organs as well as elevated tumor necrosis factor and interleukin-6 serum concentrations in CP-treated and S. aureus-infected mice were significantly lowered upon application of phages. The phages markedly increased the percentage of circulating neutrophils and immature cells from the myelocytic and lymphocytic lineages in CP-treated, S. aureus-infected mice as well as of myelocytes and immature neutrophils in the bone marrow. In addition, phages stimulated in such mice generation of specific agglutinins against S. aureus. Application of specific phages to immunosuppressed mice prior to infection with S. aureus proved very effective, suggesting a potential benefit of phage therapy in immunocompromised patients experiencing bacterial infections.
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影响因子：
3.1
作者：
BUHLES, WC;SHIFRINE, M
通讯作者：
SHIFRINE, M
DOI：
10.1179/096805103225001422
发表时间：
2003-01-01
期刊：
JOURNAL OF ENDOTOXIN RESEARCH
影响因子：
--
作者：
Cui, W;Lei, MG;Morrison, DC
通讯作者：
Morrison, DC
影响因子：
4
作者：
Lacki, JK;Mackiewicz, SH;Muller, W
通讯作者：
Muller, W
影响因子：
5.5
作者：
Boxio, R;Bossenmeyer-Pourié, C;Nüsse, O
通讯作者：
Nüsse, O
影响因子：
4.4
作者：
Artyrn, J;Zimecki, M;Kruzel, ML
通讯作者：
Kruzel, ML