Identification of novel tumor-associated cell surface sialoglycoproteins in human glioblastoma tumors using quantitative proteomics.
Identification of novel tumor-associated cell surface sialoglycoproteins in human glioblastoma tumors using quantitative proteomics.
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使用定量蛋白质组学鉴定人胶质母细胞瘤中新型肿瘤相关细胞表面唾液酸糖蛋白。
DOI：
10.1371/journal.pone.0110316
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
August P
中科院分区：
文献类型：
--
作者：
Autelitano F;Loyaux D;Roudières S;Déon C;Guette F;Fabre P;Ping Q;Wang S;Auvergne R;Badarinarayana V;Smith M;Guillemot JC;Goldman SA;Natesan S;Ferrara P;August P
Glioblastoma multiform (GBM) remains clinical indication with significant “unmet medical need”. Innovative new therapy to eliminate residual tumor cells and prevent tumor recurrences is critically needed for this deadly disease. A major challenge of GBM research has been the identification of novel molecular therapeutic targets and accurate diagnostic/prognostic biomarkers. Many of the current clinical therapeutic targets of immunotoxins and ligand-directed toxins for high-grade glioma (HGG) cells are surface sialylated glycoproteins. Therefore, methods that systematically and quantitatively analyze cell surface sialoglycoproteins in human clinical tumor samples would be useful for the identification of potential diagnostic markers and therapeutic targets for malignant gliomas. In this study, we used the bioorthogonal chemical reporter strategy (BOCR) in combination with label-free quantitative mass spectrometry (LFQ-MS) to characterize and accurately quantify the individual cell surface sialoproteome in human GBM tissues, in fetal, adult human astrocytes, and in human neural progenitor cells (NPCs). We identified and quantified a total of 843 proteins, including 801 glycoproteins. Among the 843 proteins, 606 (72%) are known cell surface or secreted glycoproteins, including 156 CD-antigens, all major classes of cell surface receptor proteins, transporters, and adhesion proteins. Our findings identified several known as well as new cell surface antigens whose expression is predominantly restricted to human GBM tumors as confirmed by microarray transcription profiling, quantitative RT-PCR and immunohistochemical staining. This report presents the comprehensive identification of new biomarkers and therapeutic targets for the treatment of malignant gliomas using quantitative sialoglycoproteomics with clinically relevant, patient derived primary glioma cells.
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影响因子：
6.4
作者：
Chandramohan, Vidyalakshmi;Bao, Xuhui;Kaneko, Mika Kato;Kato, Yukinari;Keir, Stephen T.;Szafranski, Scott E.;Kuan, Chien-Tsun;Pastan, Ira H.;Bigner, Darell D.
通讯作者：
Bigner, Darell D.
DOI：
10.1073/pnas.0811481106
发表时间：
2009-01-06
影响因子：
11.1
作者：
Laughlin, Scott T.;Bertozzi, Carolyn R.
通讯作者：
Bertozzi, Carolyn R.
影响因子：
3
作者：
Grassadonia, A;Tinari, N;Iacobelli, S
通讯作者：
Iacobelli, S
影响因子：
--
作者：
Deng J;Gao G;Wang L;Wang T;Yu J;Zhao Z
通讯作者：
Zhao Z
影响因子：
8.8
作者：
Auvergne RM;Sim FJ;Wang S;Chandler-Militello D;Burch J;Al Fanek Y;Davis D;Benraiss A;Walter K;Achanta P;Johnson M;Quinones-Hinojosa A;Natesan S;Ford HL;Goldman SA
通讯作者：
Goldman SA