Methods for studying mammalian aquaporin biology.

Methods for studying mammalian aquaporin biology.
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DOI:
10.1093/biomethods/bpad031
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发表时间:
2023
影响因子:
3.6
通讯作者:
--
中科院分区:
其他
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--
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水通道蛋白(Aquaporins,AQP)是一种跨膜水传导通道,在健康和疾病细胞中发挥着重要的生理作用,尤其是在生物医学领域。已经实施了许多方法来阐明水通道蛋白介导的水运输和下游信号激活在引发整个细胞,组织和器官的功能反应的参与。为了调节这些反应,已经采用其他方法来研究AQP的可药性。这篇综述讨论了标准的体外,在体内,和在硅方法研究水通道蛋白,特别是生物医学和哺乳动物细胞生物学的应用。我们还提出了一些新的技术和方法,为未来的AQP研究,以解决目前的差距,方法。
Aquaporins (AQPs), transmembrane water-conducting channels, have earned a great deal of scrutiny for their critical physiological roles in healthy and disease cell states, especially in the biomedical field. Numerous methods have been implemented to elucidate the involvement of AQP-mediated water transport and downstream signaling activation in eliciting whole cell, tissue, and organ functional responses. To modulate these responses, other methods have been employed to investigate AQP druggability. This review discusses standard in vitro, in vivo, and in silico methods for studying AQPs, especially for biomedical and mammalian cell biology applications. We also propose some new techniques and approaches for future AQP research to address current gaps in methodology.
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