Exploring cell membrane water exchange in aquaporin-4-deficient ischemic mouse brain using diffusion-weighted MRI.
Exploring cell membrane water exchange in aquaporin-4-deficient ischemic mouse brain using diffusion-weighted MRI.
复制标题
用弥散加权磁共振研究水通道蛋白-4缺陷性脑缺血小鼠脑内细胞膜的水交换。
DOI：
10.1186/s41747-021-00244-y
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发表时间：
2021-10-07
影响因子：
3.8
通讯作者：
Obata T
中科院分区：
文献类型：
--
作者：
Urushihata T;Takuwa H;Takahashi M;Kershaw J;Tachibana Y;Nitta N;Shibata S;Yasui M;Higuchi M;Obata T
水通道蛋白-4是一种在脑星形胶质细胞中高度表达的膜通道蛋白，促进水分子的转运。已经表明，抑制水通道蛋白-4功能可能是减轻脑梗死后细胞水肿的有效治疗。因此，重要的是开发临床适用的测量系统，以评估和更好地了解水通道蛋白-4抑制对活体的影响。通过手术闭塞野生型和水通道蛋白4基因敲除小鼠的大脑中动脉来建立局灶性脑缺血的动物模型，之后进行多b值多扩散时间扩散加权成像测量。采用表观扩散系数（ADC）模型和房室水交换模型对数据进行分析。估计5个不同B值范围的ADC。水通道蛋白-4敲除小鼠对侧区域的ADC在每个范围内均显著高于野生型小鼠。相比之下，水通道蛋白-4敲除小鼠在缺血组织中对于每个b值范围具有比野生型小鼠显著更低的ADC。ADC的基因型依赖性差异对于正常组织中的最低范围和缺血组织中的最高范围特别显著。两种基因型在不同扩散时间测得的ADC值差异显著。水交换模型与缺血区域数据的拟合发现，水通道蛋白-4敲除小鼠的水交换时间比野生型小鼠长约2.5倍。多b值多扩散时间弥散加权成像可能有助于水通道蛋白4相关疾病的体内研究和临床诊断。在线版本包含补充材料，可通过10.1186/s41747-021-00244-y获得。
Aquaporin-4 is a membrane channel protein that is highly expressed in brain astrocytes and facilitates the transport of water molecules. It has been suggested that suppression of aquaporin-4 function may be an effective treatment for reducing cellular edema after cerebral infarction. It is therefore important to develop clinically applicable measurement systems to evaluate and better understand the effects of aquaporin-4 suppression on the living body. Animal models of focal cerebral ischemia were created by surgically occluding the middle cerebral artery of wild-type and aquaporin-4 knockout mice, after which multi-b-value multi-diffusion-time diffusion-weighted imaging measurements were performed. Data were analyzed with both the apparent diffusion coefficient (ADC) model and a compartmental water-exchange model. ADCs were estimated for five different b value ranges. The ADC of aquaporin-4 knockout mice in the contralateral region was significantly higher than that of wild-type mice for each range. In contrast, aquaporin-4 knockout mice had significantly lower ADC than wild-type mice in ischemic tissue for each b-value range. Genotype-dependent differences in the ADC were particularly significant for the lowest ranges in normal tissue and for the highest ranges in ischemic tissue. The ADCs measured at different diffusion times were significantly different for both genotypes. Fitting of the water-exchange model to the ischemic region data found that the water-exchange time in aquaporin-4 knockout mice was approximately 2.5 times longer than that in wild-type mice. Multi-b-value multi-diffusion-time diffusion-weighted imaging may be useful for in vivo research and clinical diagnosis of aquaporin-4-related diseases. The online version contains supplementary material available at 10.1186/s41747-021-00244-y.
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影响因子：
3.3
作者：
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通讯作者：
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