Expression of aquaporin 1, 3 and 5 in colorectal carcinoma: correlation with clinicopathological characteristics and prognosis.
Expression of aquaporin 1, 3 and 5 in colorectal carcinoma: correlation with clinicopathological characteristics and prognosis.
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DOI：
10.3389/pore.2023.1611179
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发表时间：
2023
影响因子：
2.8
通讯作者：
Zhang, Jinsong
中科院分区：
文献类型：
--
作者：
Zhang, Guangwen;Hao, Yongfei;Chen, Ling;Li, Zengshan;Gao, Langlang;Tian, Jian;Qiao, Qing;Zhang, Jinsong
背景：结直肠癌（CRC）的预后生物标志物在治疗策略中具有重要作用。研究表明，水通道蛋白（AQP）的高表达与多种人类肿瘤的不良预后相关。AQP参与了CRC的发生和发展。本研究旨在探讨大肠癌组织中AQP 1、3、5的表达与临床病理特征及预后的关系。 研究方法：采用免疫组化方法检测2006年6月至2008年11月间112例大肠癌组织芯片标本中AQP 1、3、5的表达。用Quupath软件计算AQP表达评分（Allred_score和H_score）。根据最佳临界值将患者分为高表达或低表达亚组。采用卡方检验、t检验或单因素方差分析评价AQP表达与临床病理特征之间的关系。采用时间依赖ROC曲线、Kaplan-Meier曲线、单因素和多因素考克斯分析对5年无进展生存期（PFS）和总生存期（OS）进行生存分析。 结果如下：AQP 1、3和5的表达与结直肠癌的区域淋巴结转移、组织学分级和肿瘤部位有关（p < 0.05）。Kaplan-Meier曲线显示，AQP 1高表达患者的5年PFS低于AQP 1低表达患者（Allred_score：47% vs. 72%，p = 0.015; H_score：52% vs. 78%，p = 0.006）以及5年OS（Allred_score：51%对比75%，p = 0.005; H_score：56%对比80%，p = 0.002）。多因素考克斯回归分析显示，AQP 1表达是影响预后的独立危险因素（p = 0.033，HR = 2.274，HR95%CI：1.069-4.836）。AQP 3、5的表达与预后无明显相关性。 结论：AQP 1、3和5的表达与不同的临床病理特征相关，AQP 1的表达可能是大肠癌预后的一个潜在的生物标志物。
Background: Prognostic biomarkers in colorectal carcinoma (CRC) have an important role in therapeutic strategy. Studies have shown that high expression of Aquaporin (AQP) is associated with poor prognosis in a variety of human tumors. AQP is involved in the initiation and development of CRC. The present study aimed to investigate the correlation between the expression of AQP1, 3 and 5 and clinicopathological features or prognosis in CRC. Methods: The AQP1, 3 and 5 expressions were analyzed based on the immunohistochemical staining of tissue microarray specimens including 112 patients with CRC between June 2006 and November 2008. The expression score of AQP (Allred_score and H_score) was digitally obtained with Qupath software. Patients were divided into high or low expression subgroups based on the optimal cut-off values. The relationship between expression of AQP and clinicopathological characteristics were evaluated using chi-square test, t-test, or one-way ANOVA, when appropriate. Survival analysis of 5-year progression free survival (PFS) and overall survival (OS) was performed with time-dependent ROC, Kaplan-Meier curves, univariate and multivariate COX analysis. Results: The AQP1, 3 and 5 expressions were associated with regional lymph node metastasis, histological grading, and tumor location in CRC, respectively (p < 0.05). Kaplan-Meier curves showed that patients with high AQP1 expression had worse 5-year PFS than those with low AQP1 expression (Allred_score: 47% vs. 72%, p = 0.015; H_score: 52% vs. 78% p = 0.006), as well as 5-year OS (Allred_score: 51% vs. 75%, p = 0.005; H_score: 56% vs. 80%, p = 0.002). Multivariate Cox regression analysis indicated that AQP1 expression was an independent risk prognostic factor (p = 0.033, HR = 2.274, HR95% CI: 1.069–4.836). There was no significant correlation between the expression of AQP3 and 5 and the prognosis. Conclusion: The AQP1, 3 and 5 expressions correlate with different clinicopathological characteristics and the AQP1 expression may be a potential biomarker of prognosis in CRC.
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影响因子：
5.6
作者：
Marlar S;Jensen HH;Login FH;Nejsum LN
通讯作者：
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Zitvogel, Laurence