Cellular localization of aquaporin-1 in the human and mouse trigeminal systems.
Cellular localization of aquaporin-1 in the human and mouse trigeminal systems.
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DOI：
10.1371/journal.pone.0046379
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Xiao M
中科院分区：
文献类型：
--
作者：
Gao J;Tan M;Gu M;Marshall C;Ding J;Hu G;Xiao M
以往的研究报道，小鼠和大鼠三叉神经细胞亚群表达水通道水通道蛋白-1(AQP1)。在本研究中，我们对AQP1在人和小鼠三叉神经系统中的定位进行了比较研究。免疫组织化学和免疫荧光结果显示，AQP1定位于部分中、小型三叉神经细胞的胞浆和细胞膜。此外，在人和小鼠的三叉神经外周轴突中也发现了AQP1。在三叉神经中央根和脑干，AQP1在人的星形胶质细胞中特异表达，而在小鼠的三叉神经中央根和三叉神经脊束核中仅有神经纤维表达。此外，AQP1阳性神经纤维存在于人和小鼠口腔组织的粘膜层和粘膜下层，但不存在于肌肉层和皮下组织。荧光金逆行示踪显示，AQP1阳性三叉神经细胞分布于颊粘膜，但不分布于颊皮肤。这些结果表明，AQP1在人和小鼠三叉神经系统中的细胞定位既有相似之处，也有不同之处。AQP1在支配口腔粘膜的三叉神经细胞中的选择性表达表明AQP1参与了口腔感觉转导。
Previous studies reported that a subpopulation of mouse and rat trigeminal neurons express water channel aquaporin-1 (AQP1). In this study we make a comparative investigation of AQP1 localization in the human and mouse trigeminal systems. Immunohistochemistry and immunofluorescence results showed that AQP1 was localized to the cytoplasm and cell membrane of some medium and small-sized trigeminal neurons. Additionally, AQP1 was found in numerous peripheral trigeminal axons of humans and mice. In the central trigeminal root and brain stem, AQP1 was specifically expressed in astrocytes of humans, but was restricted to nerve fibers within the central trigeminal root and spinal trigeminal tract and nucleus in mice. Furthermore, AQP1 positive nerve fibers were present in the mucosal and submucosal layers of human and mouse oral tissues, but not in the muscular and subcutaneous layers. Fluorogold retrograde tracing demonstrated that AQP1 positive trigeminal neurons innervate the mucosa but not skin of cheek. These results reveal there are similarities and differences in the cellular localization of AQP1 between the human and mouse trigeminal systems. Selective expression of AQP1 in the trigeminal neurons innervating the oral mucosa indicates an involvement of AQP1 in oral sensory transduction.
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影响因子：
3.3
作者：
Li, X.;Kong, H.;Hu, G.
通讯作者：
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DOI：
10.1097/nen.0b013e3181fd252c
发表时间：
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作者：
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