Transgenerational stress memory is not a general response in Arabidopsis.
Transgenerational stress memory is not a general response in Arabidopsis.
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DOI：
10.1371/journal.pone.0005202
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发表时间：
2009
期刊：
影响因子：
3.7
通讯作者：
Mittelsten Scheid O
中科院分区：
文献类型：
--
作者：
Pecinka A;Rosa M;Schikora A;Berlinger M;Hirt H;Luschnig C;Mittelsten Scheid O
不利的条件会在植物体内引发DNA损伤和DNA修复反应。众所周知，各种应激因素通过增加必需酶组分的浓度和事件的频率来刺激同源重组，这是最准确的修复途径。据报道，这种影响会持续到没有暴露在压力下的后代。为了为这种跨代应激记忆的遗传分析奠定基础，我们测试了一系列处理对后代同源重组的定量影响。几个转有成熟重组陷阱的拟南芥品系被暴露于10种不同的物理和化学胁迫处理中，并对处理世代和未处理的后续两代中的体细胞同源重组(SHR)事件的数量进行了评分。这些数字与同源重组和修复相关基因的表达水平有关。在大多数治疗后，SHR得到了增强，证实了先前的数据，并增加了新的有效应激类型，特别是与染色质的干扰。直接修饰DNA的化合物刺激SHR的值超过前面描述的诱导率，伴随着涉及SHR的基因的诱导。尽管在应激的世代中有显著的刺激，但随后的两代未处理的SHR仅显示出低和随机的增加，与亲本植物中的刺激程度无关。在后代中，SHR酶的编码转录本通常恢复到处理前的水平。因此，对SHR频率的跨代效应不是拟南芥对非生物胁迫的一般反应，可能需要特殊的条件。
Adverse conditions can trigger DNA damage as well as DNA repair responses in plants. A variety of stress factors are known to stimulate homologous recombination, the most accurate repair pathway, by increasing the concentration of necessary enzymatic components and the frequency of events. This effect has been reported to last into subsequent generations not exposed to the stress. To establish a basis for a genetic analysis of this transgenerational stress memory, a broad range of treatments was tested for quantitative effects on homologous recombination in the progeny. Several Arabidopsis lines, transgenic for well-established recombination traps, were exposed to 10 different physical and chemical stress treatments, and scored for the number of somatic homologous recombination (SHR) events in the treated generation as well as in the two subsequent generations that were not treated. These numbers were related to the expression level of genes involved in homologous recombination and repair. SHR was enhanced after the majority of treatments, confirming previous data and adding new effective stress types, especially interference with chromatin. Compounds that directly modify DNA stimulated SHR to values exceeding previously described induction rates, concomitant with an induction of genes involved in SHR. In spite of the significant stimulation in the stressed generations, the two subsequent non-treated generations only showed a low and stochastic increase in SHR that did not correlate with the degree of stimulation in the parental plants. Transcripts coding for SHR enzymes generally returned to pre-treatment levels in the progeny. Thus, transgenerational effects on SHR frequency are not a general response to abiotic stress in Arabidopsis and may require special conditions.
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