Fluorine-Nitrogen-Codoped Carbon Dots as Fluorescent Switch Probes for Selective Fe(III) and Ascorbic Acid Sensing in Living Cells.
Fluorine-Nitrogen-Codoped Carbon Dots as Fluorescent Switch Probes for Selective Fe(III) and Ascorbic Acid Sensing in Living Cells.
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DOI：
10.3390/molecules27196158
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发表时间：
2022-09-20
期刊：
影响因子：
--
通讯作者：
Li H
中科院分区：
文献类型：
--
作者：
Ye S;Zhang M;Guo J;Yu X;Song J;Zeng P;Qu J;Chen Y;Li H
基于碳点的高质量荧光探针具有稳定性好、毒性低、量子产率高、原料价格低廉等优点，在许多领域具有广阔的应用前景。以3-氨基苯酚和2，4-二氟苯甲酸为原料，采用水热法成功地合成了具有蓝色荧光的氟氮双掺杂硫化镉(NFCDS)。该探针可直接或间接检测Fe~(3+)(检测范围：0.1~150μM，检出限：0.14μM)和抗坏血酸(AA，检测范围：10~80μM，检出限：0.11μM)。基于NFCDS的探针对Fe3+和抗坏血酸(AA)表现出极高的选择性和很强的抗干扰性。此外，我们在活细胞中检测了NFCDs对Fe3+和AA的反应，这表明及时使用AA可以减轻铁中毒的影响。这具有重要的生物学意义。这意味着使用NFCDs作为荧光探针有利于检测Fe3+和AA，并观察其在活细胞中的动态变化。因此，这项工作可能有助于Fe3+和AA相关疾病的研究。
High-quality fluorescent probes based on carbon dots (CDs) have promising applications in many fields owing to their good stability, low toxicity, high quantum yield, and low raw material price. The fluorine- and nitrogen-doped fluorescent CDs (NFCDs) with blue fluorescence was successfully synthesized using 3-aminophenol and 2,4-difluorobenzoic acid as the raw material by the hydrothermal method. The NFCDs as probe can be used to directly and indirectly detect Fe3+ (detection range: 0.1–150 μM and detection limit: 0.14 μM) and ascorbic acid (AA) (detection range: 10–80 μM and detection limit: 0.11 μM). The NFCDs-based probe shows exceptional selectivity and strong anti-interference for Fe3+ and ascorbic acid (AA). In addition, we examined the response of NFCDs to Fe3+ and AA in living cells, which showed that the timely use of AA can reduce the effects of iron poisoning. This has important biological significance. This means that using NFCDs as fluorescent probes is beneficial for Fe3+ and AA detection and observing their dynamic changes in living cells. Thus, this work may contribute to the study of Fe3+- and AA-related diseases.
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影响因子：
9.9
作者：
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通讯作者：
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作者：
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作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
6.1
作者：
Ma, Y.;Chen, A. Y.;Li, Y.
通讯作者：
Li, Y.