Sex differences in diazepam effects and parvalbumin-positive GABA neurons in trait anxiety Long Evans rats.
Sex differences in diazepam effects and parvalbumin-positive GABA neurons in trait anxiety Long Evans rats.
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DOI：
10.1016/j.bbr.2014.04.048
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发表时间：
2014-08-15
影响因子：
2.7
通讯作者：
Donaldson, S. Tiffany
中科院分区：
文献类型：
--
作者：
Ravenelle, Rebecca;Neugebauer, Nichole M.;Niedzielak, Timothy;Donaldson, S. Tiffany
在临床人群中，许多焦虑症的患病率在男性和女性之间存在差异，性腺激素被认为是造成这些差异的原因。虽然在一些模型中，这些激素已被证明可以调节苯二氮卓类激动剂地西泮的抗焦虑作用，但研究结果并不一致。在这里，我们测试了在高海拔迷宫中表现出高（HAn）和低（LAn）焦虑样行为的雄性和雌性大鼠在30分钟地西泮（1.0 mg/kg）预处理后对焦虑性刺激的反应的性别差异。急性地西泮仅在汉族男性中导致焦虑样行为的减少，这可以通过在升高+迷宫（EPM）中打开手臂时间的百分比显着增加来证明。对中央杏仁核（CeA）、尾状壳核（CPu）和海马的小白蛋白（PV，一种选择性染色gaba能神经元的钙结合蛋白）的免疫组织化学分析表明，这些区域gaba能中间神经元的数量因性别和焦虑特征而异。在中央处理器中，雌性比雄性有更多的pv免疫反应（IR）细胞，LAn雌性比HAn雌性有更多的PV-IR神经元。在CeA中，与雌性相比，雄性显示出更多的PV-IR神经元，而LAn和HAn之间没有差异。此外，无论性别，海马体CA2区域的性状差异都很明显。综上所述，这些数据表明性腺激素和特质焦虑可能影响对地西泮抗焦虑作用的敏感性，这些差异可能部分是由于含有gaba的中间神经元的分布。
In clinical populations, prevalence rates for a number of anxiety disorders differ between males and females and gonadal hormones are thought to contribute to these differences. While these hormones have been shown to modulate the anxiolytic effects of the benzodiazepine agonist diazepam in some models, findings are inconsistent. Here, we tested for sex differences in response to anxiogenic stimuli following a 30-min diazepam (1.0 mg/kg) pre-treatment in male and female rats showing high (HAn) and low (LAn) anxiety-like behavior on the elevated plus maze. Acute diazepam administration resulted in decreased anxiety-like behavior only in HAn males as demonstrated by a significant increase in percent open arm time in the elevated plus maze (EPM). Immunohistochemical analysis for parvalbumin (PV; a calcium-binding protein that selectively stains GABAergic neurons) in central amygdala (CeA), caudate putamen (CPu) and the hippocampus indicated the number of GABAergic interneurons in these areas differed across sex and anxiety trait. In the CPu, females had significantly more PV-immunoreactive (IR) cells than males, and LAn females had greater PV-IR neurons than HAn females. In the CeA, males displayed an increased number of PV-IR neurons compared to females, with no differences found between LAn and HAn. Further, trait differences were evident in the CA2 region of the hippocampus, regardless of sex. Taken together, these data suggest that gonadal hormones and trait anxiety may influence the sensitivity to the anti-anxiety effects of diazepam and these differences may be due in part to the distribution of GABA-containing interneurons.
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影响因子：
16.2
作者：
Marowsky, A;Yanagawa, Y;Vogt, KE
通讯作者：
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