Expression of vascular endothelial growth factor C and vascular endothelial growth factor receptor 3 in corneal lymphangiogenesis.
Expression of vascular endothelial growth factor C and vascular endothelial growth factor receptor 3 in corneal lymphangiogenesis.
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血管内皮生长因子C和血管内皮生长因子受体3在角膜淋巴管生成中的表达。
DOI：
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发表时间：
2001
影响因子：
3.4
通讯作者：
Yasuo Ishii
中科院分区：
文献类型：
--
作者：
T. Mimura;S. Amano;T. Usui;Y. Kaji;T. Oshika;Yasuo Ishii
血管化的角膜有淋巴管生成的报道。然而，角膜淋巴管生成的分子机制尚不清楚。由于淋巴管可能通过加速抗原识别和移植物排斥而导致血管化角膜移植成功率降低，阐明角膜淋巴管生成的机制将有助于抑制淋巴管，并可能改善角膜移植的结果。本研究旨在检测血管内皮生长因子- c （VEGF-C）及其受体之一血管内皮生长因子受体-3 （VEGFR-3）在角膜淋巴管生成中的表达。VEGF-C是迄今为止报道的唯一内源性淋巴管生成因子。采用硝酸银诱导大鼠角膜周向新生血管形成模型。电镜观察大鼠角膜损伤后淋巴管的存在。采用竞争性反转录聚合酶链反应（RT-PCR）定量角膜VEGF-C和VEGFR-3 mRNA水平，免疫组织化学原位分析VEGF-C和VEGFR-3蛋白水平。电镜显示血管化大鼠角膜内有淋巴管。竞争性RT-PCR显示，VEGF-C mRNA在大鼠角膜中正常表达缺失，在损伤后3天显著上调，并逐渐降低。大鼠角膜中VEGFR-3的表达在损伤前最低，损伤后3、7 d表达上调。损伤后2周和4周也很少被发现。在大鼠角膜损伤后3天的免疫组化分析中，VEGF-C主要在炎症细胞中检测到，vegf -3在角膜基质的几条新血管中检测到。这些数据提示VEGF-C和VEGFR-3是角膜淋巴管生成病理生理相关的内源性因子。
Lymphangiogenesis has been reported in vascularized corneas. However, the molecular mechanisms of lymphangiogenesis in the cornea are still unclear. Since lymphatic vessels may contribute to a decreased success rate of keratoplasty in vascularized cornea by accelerating antigen recognition and graft rejection, elucidation of the mechanisms of corneal lymphangiogenesis will facilitate the inhibition of lymphatic vessels and may improve the outcome of keratoplasty. This study aimed to examine the expression of vascular endothelial growth factor-C (VEGF-C), which is the only endogenous lymphangiogenic factor reported so far, and one of its receptors, vascular endothelial growth factor receptor-3 (VEGFR-3), in corneal lymphangiogenesis. A rat model was used in which silver nitrate application resulted in corneal circumferential neovascularization. The presence of lymphatic vessels in the rat injured cornea was examined with electron microscope. Corneal VEGF-C and VEGFR-3 mRNA levels were quantified with competitive reverse transcription polymerase chain reaction (RT-PCR), and VEGF-C and VEGFR-3 proteins were studied in situ using immunohistochemical analysis. Electron microscopy revealed lymphatic vessels in the vascularized rat corneas. Competitive RT-PCR demonstrated that the expression of VEGF-C mRNA in the rat cornea was normally absent, and was dramatically up-regulated 3 days after the injury, which gradually decreased. The VEGFR-3 expression in the rat cornea was minimally detected before the injury and was up-regulated 3 and 7 days after the injury. It was also minimally detected 2 and 4 weeks after the injury. In immunohistochemical analysis of the rat cornea 3 days after the injury, VEGF-C was mainly detected in inflammatory cells, and VEGFR-3 was demonstrated in several new vessels in the corneal stroma. These data suggest that VEGF-C and VEGFR-3 are pathophysiologically relevant endogenous factors in corneal lymphangiogenesis.
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影响因子：
4.4
作者：
Ivana K. Kim;A. Ryan;R. Rohan;S. Amano;Stephanie Agular;Joan W. Miller;A. Adamis
通讯作者：
Ivana K. Kim;A. Ryan;R. Rohan;S. Amano;Stephanie Agular;Joan W. Miller;A. Adamis
影响因子：
56.9
作者：
DEVRIES, C;ESCOBEDO, JA;WILLIAMS, LT
通讯作者：
WILLIAMS, LT
影响因子：
2.7
作者：
Oh, SJ;Jeltsch, MM;Wilting, J
通讯作者：
Wilting, J
影响因子：
2.7
作者：
Wittwer, CT;Ririe, KM;Balis, UJ
通讯作者：
Balis, UJ
化学烧灼诱发角膜新生血管的放射自显影研究。
DOI：
--
发表时间：
1981
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
Burger,PC;Klintworth,GK
通讯作者：
Klintworth,GK