A 24-hour temporal profile of in vivo brain and heart pet imaging reveals a nocturnal peak in brain 18F-fluorodeoxyglucose uptake.
A 24-hour temporal profile of in vivo brain and heart pet imaging reveals a nocturnal peak in brain 18F-fluorodeoxyglucose uptake.
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DOI：
10.1371/journal.pone.0031792
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Duffield GE
中科院分区：
文献类型：
--
作者：
van der Veen DR;Shao J;Chapman S;Leevy WM;Duffield GE
使用正电子发射断层扫描，我们测量了在体内摄取的18F-氟脱氧葡萄糖（FDG）在大脑和心脏的C57 B1/6小鼠在整个24小时的光暗周期的时间间隔。我们的数据描述了一个显着的，高幅度的节奏，在FDG摄取整个大脑，在中黑暗阶段的光-暗周期，这是夜间小鼠的活动阶段达到峰值。在这些条件下，心脏FDG摄取不随一天中的时间而变化，但在同一小鼠内的24小时测量中确实显示出生物学变化。FDG摄取在一天中的不同时间在单个小鼠内进行扫描，并且还与个体之间的一天中的不同时间进行比较，显示FDG摄取的24小时模式的生物学和技术再现性。脑FDG摄取的区域分析显示，特别是高振幅的节奏在嗅球和皮层，而低振幅的节奏，观察杏仁核，脑干和下丘脑。局部FDG摄取的低振幅24小时节律可能是由于单个脑结构中具有不同相位的多个节律，从而淬灭了一些振幅。我们的数据表明，整个大脑表现出显着的，高幅度的日常变化，在活小鼠的葡萄糖摄取。应用2-脱氧-D [14 C]-葡萄糖方法定量测定局部脑葡萄糖利用率的报告表明，只有少数脑区显示葡萄糖利用的昼夜变化。相比之下，我们的数据显示了大多数大脑区域的葡萄糖摄取的24小时模式，包括几个在葡萄糖利用方面没有表现出差异的区域。我们的数据还强调了在临床和临床前环境中控制大脑中FDG摄取扫描的时间的方法学要求，并建议在一天中的不同时间进行FDG测量的波形标准化。
Using positron emission tomography, we measured in vivo uptake of 18F-fluorodeoxyglucose (FDG) in the brain and heart of C57Bl/6 mice at intervals across a 24-hour light-dark cycle. Our data describe a significant, high amplitude rhythm in FDG uptake throughout the whole brain, peaking at the mid-dark phase of the light-dark cycle, which is the active phase for nocturnal mice. Under these conditions, heart FDG uptake did not vary with time of day, but did show biological variation throughout the 24-hour period for measurements within the same mice. FDG uptake was scanned at different times of day within an individual mouse, and also compared to different times of day between individuals, showing both biological and technical reproducibility of the 24-hour pattern in FDG uptake. Regional analysis of brain FDG uptake revealed especially high amplitude rhythms in the olfactory bulb and cortex, while low amplitude rhythms were observed in the amygdala, brain stem and hypothalamus. Low amplitude 24-hour rhythms in regional FDG uptake may be due to multiple rhythms with different phases in a single brain structure, quenching some of the amplitude. Our data show that the whole brain exhibits significant, high amplitude daily variation in glucose uptake in living mice. Reports applying the 2-deoxy-D[14C]-glucose method for the quantitative determination of the rates of local cerebral glucose utilization indicate only a small number of brain regions exhibiting a day versus night variation in glucose utilization. In contrast, our data show 24-hour patterns in glucose uptake in most of the brain regions examined, including several regions that do not show a difference in glucose utilization. Our data also emphasizes a methodological requirement of controlling for the time of day of scanning FDG uptake in the brain in both clinical and pre-clinical settings, and suggests waveform normalization of FDG measurements at different times of the day.
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