Injectable PolyMIPE Scaffolds for Soft Tissue Regeneration.
Injectable PolyMIPE Scaffolds for Soft Tissue Regeneration.
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DOI：
10.1016/j.polymer.2013.09.009
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发表时间：
2014-01-14
期刊：
影响因子：
4.6
通讯作者：
Cosgriff-Hernandez, Elizabeth
中科院分区：
文献类型：
--
作者：
Moglia, Robert S.;Robinson, Jennifer L.;Muschenborn, Andrea D.;Touchet, Tyler J.;Maitland, Duncan J.;Cosgriff-Hernandez, Elizabeth
由创伤、烧伤、手术或疾病引起的损伤通常导致软组织损失，从而导致功能受损和永久性畸形。组织工程旨在通过制造具有必要特性和生物活性线索的合成支架来克服缺乏可行的供体组织来再生这些组织。设计成与软组织模量和强度相匹配的生物材料支架还应保持组织的弹性和抗疲劳特性。特别重要的设计是支架的互连多孔结构，其需要通过促进质量运输来支持组织生长。对于缺乏脉管系统以提供营养流的新植入支架，充分的质量运输尤其如此。常见的支架制造策略通常利用有毒溶剂和高温或高压来实现所需的孔隙率。在这项研究中，聚合介质内相乳液（polyMIPE）用于生成可注射移植物，该移植物在体温下固化成多孔泡沫，而无需有毒溶剂。这些聚（酯氨基甲酸酯脲）支架具有弹性体性质，具有可调的压缩模量（20-200 kPa）和强度（4-60 kPa），以及在第一次调节循环后的高恢复率（97-99%）。所得的孔结构与由水模板孔（50-300 μm）包围的二氧化碳生成的大空隙（0.5-2 mm）高度互连。证明了通过改变乳液化学来调节支架孔结构和机械性能的能力。通过实验测量渗透率和形状因子以确定聚MIPE组合物对孔互连性的影响。最后，最初的人间充质干细胞（hMSC）细胞相容性测试支持在再生应用中使用这些候选支架。总的来说，这些可注射的聚MIPE泡沫显示出作为软组织修复的生物材料支架的强大前景。
Injury caused by trauma, burns, surgery, or disease often results in soft tissue loss leading to impaired function and permanent disfiguration. Tissue engineering aims to overcome the lack of viable donor tissue by fabricating synthetic scaffolds with the requisite properties and bioactive cues to regenerate these tissues. Biomaterial scaffolds designed to match soft tissue modulus and strength should also retain the elastomeric and fatigue-resistant properties of the tissue. Of particular design importance is the interconnected porous structure of the scaffold needed to support tissue growth by facilitating mass transport. Adequate mass transport is especially true for newly implanted scaffolds that lack vasculature to provide nutrient flux. Common scaffold fabrication strategies often utilize toxic solvents and high temperatures or pressures to achieve the desired porosity. In this study, a polymerized medium internal phase emulsion (polyMIPE) is used to generate an injectable graft that cures to a porous foam at body temperature without toxic solvents. These poly(ester urethane urea) scaffolds possess elastomeric properties with tunable compressive moduli (20–200 kPa) and strengths (4–60 kPa) as well as high recovery after the first conditioning cycle (97–99%). The resultant pore architecture was highly interconnected with large voids (0.5–2 mm) from carbon dioxide generation surrounded by water-templated pores (50–300 μm). The ability to modulate both scaffold pore architecture and mechanical properties by altering emulsion chemistry was demonstrated. Permeability and form factor were experimentally measured to determine the effects of polyMIPE composition on pore interconnectivity. Finally, initial human mesenchymal stem cell (hMSC) cytocompatibility testing supported the use of these candidate scaffolds in regenerative applications. Overall, these injectable polyMIPE foams show strong promise as a biomaterial scaffold for soft tissue repair.
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DOI：
10.1115/1.1318906
发表时间：
2000-12-01
影响因子：
1.7
作者：
Butler, DL;Goldstein, SA;Guilak, F
通讯作者：
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影响因子：
5.5
作者：
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通讯作者：
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DOI：
10.2118/4706-pa
发表时间：
1974-01-01
期刊：
SOCIETY OF PETROLEUM ENGINEERS JOURNAL
影响因子：
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作者：
GEERTSMA, J
通讯作者：
GEERTSMA, J
影响因子：
2.8
作者：
AKIZUKI, S;MOW, VC;MANICOURT, DH
通讯作者：
MANICOURT, DH
影响因子：
4.6
作者：
Benhardt, Hugh;Sears, Nick;Cosgriff-Hernandez, Elizabeth
通讯作者：
Cosgriff-Hernandez, Elizabeth