In vivo, label-free, three-dimensional quantitative imaging of kidney microcirculation using Doppler optical coherence tomography.
In vivo, label-free, three-dimensional quantitative imaging of kidney microcirculation using Doppler optical coherence tomography.
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DOI：
10.1038/labinvest.2011.112
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发表时间：
2011-11
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影响因子：
--
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中科院分区：
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多普勒光学相干层析成像(DOCT)是光学相干层析成像(OCT)的功能扩展，目前正被用于多个临床领域来定量体内的血流。在这项研究中，目的是探讨DOCT成像肾脏微循环的可行性，特别是肾小球血流。DOCT能够实时捕获由一系列横断面图像组成的3D数据集，从而实现对肾小球血流的无标记和非破坏性量化。成年雄性慕尼黑-Wistar大鼠麻醉后，通过剖腹手术暴露肾脏。肾脏在Doct显微镜下暴露后，观察肾小球血流。同时观察急性甘露醇和血管紧张素II输注的疗效。对诱导的生理状态的肾小球血流量进行量化，并与基线测量进行比较。根据3DOCT/DOCT数据集计算肾小球体积、累积多普勒体积和多普勒血流范围参数。肾小球大小从OCT开始测定，DOCT很容易显示肾小球血流。注射甘露醇后，血流显著增加，血管紧张素II注射后，血流显著减少。此外，还绘制了血流直方图，以说明不同诱导生理状态下血流速度和血容量的差异。我们在活体内演示了大鼠肾小球微循环的3D DOCT成像。在改变的生理条件下检测到血流的动态变化，证明了DOCT的实时成像能力。这种方法有望实现肾血流的非侵入性成像，用于移植肾评估或监测与疾病进展相关的肾脏血流动力学改变。
Doppler optical coherence tomography (DOCT) is a functional extension of optical coherence tomography (OCT) and is currently being employed in several clinical arenas to quantify blood flow in vivo. In this study, the objective was to investigate the feasibility of DOCT to image kidney microcirculation, specifically, glomerular blood flow. DOCT is able to capture 3D data sets consisting of a series of cross-sectional images in real time, which enables label-free and non-destructive quantification of glomerular blood flow. The kidneys of adult, male Munich-Wistar rats were exposed through laparotomy procedure after being anesthetized. Following exposure of the kidney beneath the DOCT microscope, glomerular blood flow was observed. The effects of acute mannitol and angiotensin II infusion were also observed. Glomerular blood flow was quantified for the induced physiological states and compared with baseline measurements. Glomerular volume, cumulative Doppler volume, and Doppler flow range parameters were computed from 3D OCT/DOCT data sets. Glomerular size was determined from OCT, and DOCT readily revealed glomerular blood flow. After infusion of mannitol, a significant increase in blood flow was observed and quantified, and following infusion of angiontensin II, a significant decrease in blood flow was observed and quantified. Also, blood flow histograms were produced to illustrate differences in blood flow rate and blood volume among the induced physiological states. We demonstrated 3D DOCT imaging of rat kidney microcirculation in the glomerulus in vivo. Dynamic changes in blood flow were detected under altered physiological conditions demonstrating the real-time imaging capability of DOCT. This method holds promise to allow non-invasive imaging of kidney blood flow for transplant graft evaluation or monitoring of altered renal hemodynamics related to disease progression.
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影响因子：
4.7
作者：
Hariri LP;Bonnema GT;Schmidt K;Winkler AM;Korde V;Hatch KD;Davis JR;Brewer MA;Barton JK
通讯作者：
Barton JK
影响因子：
3.4
作者：
Barton, JK;Izatt, JA;Welch, AJ
通讯作者：
Welch, AJ
影响因子：
3.8
作者：
Dunkers, JP;Cicerone, MT;Washburn, NR
通讯作者：
Washburn, NR
DOI：
10.1016/j.mric.2008.07.001
发表时间：
2008-11
影响因子：
1.6
作者：
Bokacheva, Louisa;Rusinek, Henry;Zhang, Jeff L.;Lee, Vivian S.
通讯作者：
Lee, Vivian S.
影响因子：
--
作者：
ANDREWS, PM;PETROLL, WM;JESTER, JV
通讯作者：
JESTER, JV