Dispersion of single-walled carbon nanotubes by a natural lung surfactant for pulmonary in vitro and in vivo toxicity studies.
Dispersion of single-walled carbon nanotubes by a natural lung surfactant for pulmonary in vitro and in vivo toxicity studies.
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DOI：
10.1186/1743-8977-7-31
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发表时间：
2010-10-19
影响因子：
10
通讯作者：
Rojanasakul Y
中科院分区：
文献类型：
--
作者：
Wang L;Castranova V;Mishra A;Chen B;Mercer RR;Schwegler-Berry D;Rojanasakul Y
越来越多的证据表明，纳米粒子的分散程度对其生物活性有很大的影响。本研究的目的是开发一种使用天然肺表面活性剂的简单快速的纳米颗粒分散方法，并评估单壁碳纳米管的分散状态对体外和体内细胞毒性和致纤维化性的影响。天然肺表面活性剂Survanta®用于将单壁碳纳米管（SWCNT）分散在生物介质中。在生理相关浓度下，Survanta®产生良好分散的SWCNT，而不会引起细胞毒性或纤维化效应。体外研究表明，Survanta®分散的SWCNT（SD-SWCNT）在低剂量（0.04-0.12 μg/ml或0.02-0.06 μg/cm 2暴露表面积）下刺激肺上皮细胞增殖，但在高剂量下具有抑制作用。非分散单壁碳纳米管（ND-SWCNT）没有表现出这些影响，表明分散状态的单壁碳纳米管的生物活性的重要性。使用培养的人肺成纤维细胞的研究表明，SD-SWCNT刺激细胞的胶原蛋白产生。该结果得到使用丙酮/超声处理分散的SWCNT（AD-SWCNT）的类似观察结果的支持，表明Survanta®不掩蔽SWCNT的生物活性。同样，体内研究表明，SD-SWCNT和AD-SWCNT均诱导小鼠肺纤维化，而单独的分散剂Survanta®或Survanta®分散的对照超细炭黑没有效果。结果表明，Survanta®可有效地将SWCNT分散在生物介质中，而不会在本研究中使用的测试浓度下引起细胞毒性效应。SD-SWCNT在体外刺激肺成纤维细胞的胶原蛋白产生并在体内诱导肺纤维化。用AD-SWCNT观察到类似的结果，支持Survanta®不掩盖SWCNT的生物活性的结论，因此可以用作有效的分散剂。由于过度的胶原蛋白的产生是肺纤维化的标志，本研究的结果表明，在体外模型使用肺成纤维细胞可能是一个有效的和快速的筛选工具，用于预测单壁碳纳米管在体内的纤维化潜力。
Accumulating evidence indicate that the degree of dispersion of nanoparticles has a strong influence on their biological activities. The aims of this study were to develop a simple and rapid method of nanoparticle dispersion using a natural lung surfactant and to evaluate the effect of dispersion status of SWCNT on cytotoxicity and fibrogenicity in vitro and in vivo. The natural lung surfactant Survanta® was used to disperse single-walled carbon nanotubes (SWCNT) in a biological medium. At physiologically relevant concentrations, Survanta® produced well dispersed SWCNT without causing a cytotoxic or fibrogenic effect. In vitro studies show that Survanta®-dispersed SWCNT (SD-SWCNT) stimulated proliferation of lung epithelial cells at low doses (0.04-0.12 μg/ml or 0.02-0.06 μg/cm2 exposed surface area) but had a suppressive effect at high doses. Non-dispersed SWCNT (ND-SWCNT) did not exhibit these effects, suggesting the importance of dispersion status of SWCNT on bioactivities. Studies using cultured human lung fibroblasts show that SD-SWCNT stimulated collagen production of the cells. This result is supported by a similar observation using Acetone/sonication dispersed SWCNT (AD-SWCNT), suggesting that Survanta® did not mask the bioactivity of SWCNT. Likewise, in vivo studies show that both SD-SWCNT and AD-SWCNT induced lung fibrosis in mice, whereas the dispersing agent Survanta® alone or Survanta®-dispersed control ultrafine carbon black had no effect. The results indicate that Survanta® was effective in dispersing SWCNT in biological media without causing cytotoxic effects at the test concentrations used in this study. SD-SWCNT stimulated collagen production of lung fibroblasts in vitro and induced lung fibrosis in vivo. Similar results were observed with AD-SWCNT, supporting the conclusion that Survanta® did not mask the bioactivities of SWCNT and thus can be used as an effective dispersing agent. Since excessive collagen production is a hallmark of lung fibrosis, the results of this study suggest that the in vitro model using lung fibroblasts may be an effective and rapid screening tool for prediction of the fibrogenic potential of SWCNT in vivo.
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影响因子：
2.1
作者：
McKinney, Walter;Chen, Bean;Frazer, Dave
通讯作者：
Frazer, Dave
影响因子：
11.4
作者：
Jia, G;Wang, HF;Guo, XB
通讯作者：
Guo, XB
影响因子：
5
作者：
Sager, Tina M.;Porter, Dale W.;Castranova, Vincent
通讯作者：
Castranova, Vincent
影响因子：
3.5
作者：
Monteiro-Riviere, NA;Nemanich, RJ;Riviere, JE
通讯作者：
Riviere, JE
DOI：
10.1016/0005-2736(92)90066-u
发表时间：
1992-01-10
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
SCHURCH, S;BACHOFEN, H;GREEN, F
通讯作者：
GREEN, F