A novel behavioral assay for measuring cold sensation in mice.
A novel behavioral assay for measuring cold sensation in mice.
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DOI：
10.1371/journal.pone.0039765
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Gereau RW 4th
中科院分区：
文献类型：
--
作者：
Brenner DS;Golden JP;Gereau RW 4th
冷反应的行为模型是探索冷感分子机制的重要工具。为了补充目前的冷行为测定并进一步研究这些机制，我们开发了一种测量冷反应阈值的新技术，即冷足底测定。在该测定中，动物在玻璃板上适应环境，并使用压缩干冰颗粒通过玻璃对后爪施加冷刺激。从冷却的玻璃杯中撤出的潜伏期被用作啮齿动物冷反应阈值的衡量标准，干冰颗粒在玻璃上提供逐渐变冷的刺激，使撤回潜伏期值与冷反应阈值温度的粗略估计相关联。该测定对包括吗啡诱导的镇痛、弗氏完全佐剂诱导的炎性异常性疼痛和脊髓神经结扎诱导的神经性异常性疼痛等操作高度敏感。
Behavioral models of cold responses are important tools for exploring the molecular mechanisms of cold sensation. To complement the currently cold behavioral assays and allow further studies of these mechanisms, we have developed a new technique to measure the cold response threshold, the cold plantar assay. In this assay, animals are acclimated on a glass plate and a cold stimulus is applied to the hindpaw through the glass using a pellet of compressed dry ice. The latency to withdrawal from the cooled glass is used as a measure of the cold response threshold of the rodents, and the dry ice pellet provides a ramping cold stimulus on the glass that allows the correlation of withdrawal latency values to rough estimates of the cold response threshold temperature. The assay is highly sensitive to manipulations including morphine-induced analgesia, Complete Freund's Adjuvant-induced inflammatory allodynia, and Spinal Nerve Ligation-induced neuropathic allodynia.
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影响因子：
5
作者：
PIZZIKETTI, RJ;PRESSMAN, NS;ADLER, MW
通讯作者：
ADLER, MW
影响因子：
16.2
作者：
Kwan, KY;Allchorne, AJ;Corey, DP
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作者：
TILLMAN, DB;TREEDE, RD;CAMPBELL, JN
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影响因子：
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作者：
Lampert, Angelika;O'Reilly, Andrias O.;Leffler, Andreas
通讯作者：
Leffler, Andreas